NITRIC-OXIDE SYNTHASE PROTEIN AND MESSENGER-RNA ARE DISCRETELY LOCALIZED IN NEURONAL POPULATIONS OF THE MAMMALIAN CNS TOGETHER WITH NADPH DIAPHORASE
NITRIC-OXIDE SYNTHASE PROTEIN AND MESSENGER-RNA ARE DISCRETELY LOCALIZED IN NEURONAL POPULATIONS OF THE MAMMALIAN CNS TOGETHER WITH NADPH DIAPHORASE
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DOI：
10.1016/0896-6273(91)90374-9
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发表时间：
1991-10-01
期刊：
影响因子：
16.2
通讯作者：
SNYDER, SH
中科院分区：
文献类型：
--
作者：
BREDT, DS;GLATT, CE;SNYDER, SH
一氧化氮是一种自由基，最近被公认为是神经信使分子。一氧化氮合酶现已被纯化并从大脑中克隆。使用特定的抗体和寡核苷酸探针，我们具有局部的脑一氧化氮合酶来分散大鼠和灵长类动物脑中的神经元群体。一氧化氮合酶仅是神经元，其定位与大鼠和灵长类动物中的NADPH diaphorase染色绝对是一致的。
Nitric oxide is a free radical that has been recently recognized as a neural messenger molecule. Nitric oxide synthase has now been purified and molecularly cloned from brain. Using specific antibodies and oligonucleotide probes, we have localized brain nitric oxide synthase to discrete neuronal populations in the rat and primate brain. Nitric oxide synthase is exclusively neuronal, and its localization is absolutely coincident with NADPH diaphorase staining in both rat and primate.