ELECTRICAL-STIMULATION OF HYPOTHALAMUS AND LUTEINIZING-HORMONE SECRETION IN JAPANESE-QUAIL
ELECTRICAL-STIMULATION OF HYPOTHALAMUS AND LUTEINIZING-HORMONE SECRETION IN JAPANESE-QUAIL
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DOI：
10.1677/joe.0.0670431
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发表时间：
1975-01-01
影响因子：
4
通讯作者：
FOLLETT, BK
中科院分区：
文献类型：
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作者：
DAVIES, DT;FOLLETT, BK
本实验旨在研究电刺激对雄性鹌鹑下丘脑促黄体生成素（LH）分泌的影响。通过双极电极用矩形脉冲（高度500 μ A）刺激后基底下丘脑6分钟，在刺激前20分钟和刺激后2、10、20和30分钟采集血样用于LH测定。在2 min样品中观察到最高血浆浓度。在接下来的30分钟内，LH水平降至静息浓度。LH水平的相对增加是最大的性不成熟的鹌鹑和最少的光刺激去势的鸟类，虽然最高的绝对水平被认为是在去势鹌鹑。性未成熟鹌鹑、性成熟鹌鹑和去势鹌鹑的LH增加幅度之间没有统计学差异。然后用较小的电流（200 μ A）刺激下丘脑的各个区域仅2分钟。刺激结节下丘脑（漏斗状核复合体的后背部分，PD-INC）或视前区（POR）后LH显著升高，而距离这些区域0.5 - 1.5mm的刺激不改变LH分泌。刺激前基底下丘脑，或视交叉上区，引起LH浓度的显着上升，虽然这是小于刺激后看到的POR。如果结节性下丘脑在几天前已经通过手术去传入，则POR或PD-INC的刺激无效。这些数据补充了电解损伤破坏PD-INC或POR可阻断光周期诱导的睾丸生长和LH分泌的研究。
Experiments were undertaken to localize those hypothalamic areas in the male quail (Coturnix coturnix japonica) where electrical stimulation would increase luteinizing hormone [LH] secretion. The posterior basal hypothalamus was stimulated with rectangular pulses (height 500 .mu.A) through a bipolar electrode for 6 min, blood samples being taken for LH assay 20 min before, and 2, 10, 20 and 30 min after stimulation. The highest plasma concentration was observed in the 2 min sample. Over the next 30 min the LH level decreased to the resting concentration. The relative increase in LH level was greatest in sexually immature quail and least in photostimulated castrated birds, although the highest absolute levels were seen in the castrated quail. There were no statistical differences between the magnitude of the LH increases in sexually immature, mature and castrated quail. Various hypothalamic regions were then stimulated with a smaller current (200 .mu.A) applied for only 2 min. A highly significant rise in LH followed stimulation of either the tuberal hypothalamus (postero-dorsal part of the infundibular nuclear complex, PD-INC), or the preoptic region (POR) while stimulation 0.5-1.5 mm away from these regions did not change LH secretion. Stimulation of the anterior basal hypothalamus, or of the suprachiasmatic area, caused a significant rise in LH concentration although this was less than that seen after stimulation of the POR. Stimulation in the POR or the PD-INC was ineffective if the tuberal hypothalamus had been deafferentated surgically some days previously. The data complement the studies in which destruction of the PD-INC or the POR by electrolytic lesions has been shown to block photoperiodically induced testicular growth and LH secretion.