Monoclonal antibody specific to signet ring cells, the vanadocytes of the tunicate, Ascidia sydneiensis samea
Monoclonal antibody specific to signet ring cells, the vanadocytes of the tunicate, Ascidia sydneiensis samea
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特异于印戒细胞、被囊动物的钒细胞、悉尼海鞘 (Ascidia sydneiensis Samea) 的单克隆抗体
DOI：
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发表时间：
1991
期刊：
影响因子：
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通讯作者：
H. Michibata
中科院分区：
文献类型：
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作者：
T. Uyama;T. Nishikata;N. Satoh;H. Michibata
已知海鞘在其血细胞中积累钒，并且有六到九种不同类型的血细胞。在这些细胞中，印戒细胞已被新鉴定为钒细胞（含钒血细胞）。我们制备了抗悉尼海鞘（Ascidia sydneiensis samea）印戒细胞的单克隆抗体，用于钒细胞的鉴定。建立了分泌印戒细胞特异性单克隆抗体的杂交瘤细胞系。免疫印迹分析表明，抗体识别的抗原是约45 kDa的单一多肽。表明印戒细胞特异性单克隆抗体可作为研究海鞘血细胞分化过程的有用标记物。
Ascidians are known to accumulate vanadium in their blood cells and have six to nine different types of blood cells. Among these cells, the signet ring cell has been newly identified as the vanadocyte (vanadium-containing blood cell). We have raised monoclonal antibodies specific to the signet ring cells of Ascidia sydneiensis samea for use in the identification of vanadocytes. A hybridoma cell line which secretes a monoclonal antibody specific to the signet ring cell has been established. Immunoblotting analysis showed that the antigen recognized by the antibody is a single polyptide of about 45 kDa. It appears that the monoclonal antibodies specific to the signet ring cells may be useful markers for studies for the process of differentiation of ascidian blood cells.
影响因子：
3.9
作者：
Lee,S;Kustin,K;Robinson,WE;Frankel,RB;Spartalian,K
通讯作者：
Spartalian,K