Demonstration of direct neurite-osteoclastic cell communication in vitro via the adrenergic receptor.
Demonstration of direct neurite-osteoclastic cell communication in vitro via the adrenergic receptor.
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DOI：
10.1254/jphs.09283fp
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发表时间：
2010
影响因子：
3.5
通讯作者：
Satoko Suga;S. Goto;A. Togari
中科院分区：
文献类型：
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作者：
Satoko Suga;S. Goto;A. Togari
目前，交感神经系统引起的骨代谢引起了人们极大的兴趣。最近，直接的神经突成骨细胞通信被证明使用体外共培养模型，包括神经突发芽小鼠上级颈神经节和MC 3 T3-E1成骨细胞样细胞。在本研究中，我们研究了是否存在直接的神经-破骨细胞通信在体外共培养模型，包括培养的小鼠上级颈神经节和小鼠破骨细胞样细胞。用NF-κ B配体的受体活化剂处理的RAW264.7细胞用作破骨细胞样细胞。我们发现，蝎毒（SV）的加入通过细胞内Ca（2+）动员引起神经突起激活，并在一个滞后期后，骨钙素Ca（2+）动员的共培养。SV没有任何直接的影响，在没有的神经突起的骨细胞。添加α（1）-肾上腺素能受体（AR）拮抗剂哌唑嗪可浓度依赖性地阻止SV神经激活导致的骨细胞激活。我们还发现，α 1肾上腺素能受体激动剂诱发骨细胞瞬时Ca（2+）动员和白细胞介素6的基因表达。这些结果表明，骨细胞活化通过骨细胞中的α（1）-AR作为对神经元活化的直接反应而发生。
There is currently great interest in the bone metabolism induced by the sympathetic nerve system. Recently, direct neurite-osteoblastic cell communication was demonstrated using an in vitro co-culture model comprising neurite-sprouting murine superior cervical ganglia and MC3T3-E1 osteoblast-like cells. In the present study, we examined whether the direct nerve-osteoclastic cell communication was present in an in vitro co-culture model comprising cultured murine superior cervical ganglia and mouse osteoclast-like cells. RAW264.7 cells treated with receptor activator of NF-kappaB ligand were used as osteoclast-like cells. We found that the addition of scorpion venom (SV) elicited neurite activation via intracellular Ca(2+) mobilization and, after a lag period, osteoclastic Ca(2+) mobilization in the co-culture. SV did not have any direct effect on the osteoclastic cells in the absence of the neurites. The addition of an alpha(1)-adrenergic receptor (AR) antagonist, prazosin, concentration-dependently prevented the osteoclastic activation that resulted as a consequence of neural activation by SV. We also found that alpha(1)-adrenergic receptor agonists evoked transient Ca(2+) mobilization and gene expression of interleukin-6 in osteoclastic cells. These results demonstrate that osteoclastic activation occurs via alpha(1)-AR in osteoclastic cells as a direct response to neuronal activation.