Monitoring of macrophage recruitment enhanced by Toll-like receptor 4 activation with MR imaging in nerve injury
Monitoring of macrophage recruitment enhanced by Toll-like receptor 4 activation with MR imaging in nerve injury
复制标题
通过 MR 成像监测神经损伤中 Toll 样受体 4 激活增强的巨噬细胞募集
DOI：
10.1002/mus.26097
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发表时间：
2018
期刊：
影响因子：
3.4
通讯作者：
Shen Jun
中科院分区：
文献类型：
--
作者：
Chen Meiwei;Zhang Xiang;Lu Liejing;Zhang Fang;Duan Xiaohui;Zheng Chushan;Chen Yueyao;Shen Jun
引言：巨噬细胞募集对于损伤后的神经再生至关重要。本研究的目的是调查是否超小超顺磁性氧化铁（USPIO）纳米粒子为基础的MRI可以用来监测增强巨噬细胞募集Toll样受体4（TLR 4）激活在nerveinjuries.Methods：大鼠接受腹腔注射脂多糖（LPS）或磷酸盐缓冲盐水（PBS）或没有注射（对照）后坐骨神经挤压伤。结果：与PBS组相比，LPS组T2加权像显示明显的低信号，挤压神经的T2值明显降低。低信号区与负载铁的巨噬细胞向受损神经的募集增强相关。讨论：USPIO增强MRI可用于通过神经损伤中TLR 4信号通路激活来监测增强的巨噬细胞募集。肌肉神经58：123-132，2018
Introduction: Macrophage recruitment is critical for nerve regeneration after an injury. The aim of this study was to investigate whether ultrasmall superparamagnetic iron oxide (USPIO) nanoparticle‐based MRI could be used to monitor the enhanced macrophage recruitment by Toll‐like receptor 4 (TLR4) activation in nerve injury.Methods: Rats received intraperitoneal injections of either lipopolysaccharide (LPS) or phosphate buffered saline (PBS) or no injection (controls) after a sciatic nerve crush injury. After intravenous injection of the USPIOs (LPS and PBS groups) or PBS (control group), MRI was performed and correlated with histological findings.Results: LPS group showed more remarkable hypointense signals on T2*‐weighted imaging and lower T2 values in the crushed nerves than PBS group. The hypointense signal areas were associated with an enhanced recruitment of iron‐loaded macrophages to the injured nerves.Discussion: USPIO‐enhanced MRI can be used to monitor the enhanced macrophage recruitment by means of TLR4 signal pathway activation in nerve injury.Muscle Nerve58: 123–132, 2018