Characterization of Monoclonal Antibodies that Recognize the Eimeria tenella Microneme Protein MIC2
Characterization of Monoclonal Antibodies that Recognize the Eimeria tenella Microneme Protein MIC2
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DOI：
10.1645/ge-1558.1
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发表时间：
2008-12-01
影响因子：
1.3
通讯作者：
Baba, Eiichiroh
中科院分区：
文献类型：
--
作者：
Sasai, Kazumi;Fetterer, Raymond H.;Baba, Eiichiroh
柔嫩艾美耳球虫（Eimeria tenella）的顶复门病原体引起球虫病，这是对家禽业具有重大经济影响的鸡的肠道疾病。顶复门的成员共享一个独特的分泌细胞器（棒状体，微丝和致密颗粒），介导宿主细胞的入侵和寄生虫空泡的形成和修改的分类。其中，微线体蛋白2位于E. tenella（EtMIC 2）在寄生虫粘附宿主细胞以启动寄生虫入侵过程中具有推定的功能。为了研究EtMIC 2在宿主寄生虫相互作用中的作用，描述了针对重组EtMIC 2蛋白产生的12种单克隆抗体（mAb）的生产和表征。在免疫荧光试验中，所有单克隆抗体均能与柔嫩乳杆菌、大肠杆菌的子孢子和裂殖子顶端复合体的分子发生反应。acervulina和E.马克西姆经Western blot分析，该单克隆抗体鉴定出其发育调控蛋白42 kDa，对应于EtMIC 2，并与F.堆型总的来说，这些邮件是用于详细研究艾美耳球虫属物种中EtMIC-2相关蛋白质的文件表征的有用工具。
The apicomplexan pathogens of Eimeria tenella cause coccidiosis, ill intestinal disease of chickens that has a major economic impact on the poultry industry. Members of Apicomplexa share an assortment of unique secretory organelles (rhoptries, micronemes, and dense granules) that mediate invasion of host cells and the formation and modification of the parasitophorous vacuole. Among these, microneme protein 2 front E. tenella (EtMIC2) has a putative function in parasite adhesion to the host cell to initiate file invasion process. To investigate the role of EtMIC2 in host parasite interactions, the production and characterization of 12 monoclonal antibodies (mabs) produced against recombinant EtMIC2 proteins is described. In an immunofluoresence assay, all mabs reacted with molecules belonging to the apical complex of sporozoites and merozoites of L tenella, E. acervulina, and E. maxima. By Western blot analysis, the mabs identified it developmentally regulated protein of 42 kDa corresponding, to EtMIC2 and cross-reacted with Proteins in developmental stages of F. acervulina. Collectively these Mails are useful tools for the detailed investigation of, file Characterization of EtMIC-2-related proteins ill Eimeria species.