CALORIMETRIC STUDIES OF FREEZE-INDUCED DEHYDRATION OF PHOSPHOLIPIDS
CALORIMETRIC STUDIES OF FREEZE-INDUCED DEHYDRATION OF PHOSPHOLIPIDS
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DOI：
10.1016/s0006-3495(93)81250-5
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发表时间：
1993-11-01
影响因子：
3.4
通讯作者：
STEPONKUS, PL
中科院分区：
文献类型：
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作者：
BRONSHTEYN, VL;STEPONKUS, PL
差示扫描量热法（DSC）被用于测定在多层二棕榈酰磷脂酰胆碱（DPPC）脂质体的水悬浮液中冻结的水量。研究是用脱水悬浮液（含12 - 20%重量的水）以及含有过量水的悬浮液（含30 - 70%重量的水）进行的。对于含水量大于或等于18%重量的悬浮液，在冷却过程中观察到两个结冰事件。第一个归因于脂质体外部冰的异质成核；第二个归因于脂质体内冰的同质成核。在初始水浓度在13%到16%重量之间的悬浮液中，只有在低于 - 40℃的温度下同质成核之后才会形成冰。在含有……的悬浮液中（句子未完整）
Differential scanning calorimetry (DSC) was used to determine the amount of water that freezes in an aqueous suspension of multilamellar dipalmitoylphosphatidylcholine (DPPC) liposomes The studies were performed with dehydrated suspensions (12-20 wt% water) and suspensions containing an excess of water (30-70 wt% water). For suspensions that contained greater-than-or-equal-to 18 wt% water, two ice-formation events were observed during cooling. The first was attributed to heterogeneous nucleation of extraliposomal ice; the second was attributed to homogeneous nucleation of ice within the liposomes. In suspensions with an initial water concentration between 13 and 16 wt%, ice formation occurred only after homogeneous nucleation at temperatures below -40-degrees-C. In suspensions containing