Multilocus sequence typing and antigen gene sequencing in the investigation of a meningococcal disease outbreak
Multilocus sequence typing and antigen gene sequencing in the investigation of a meningococcal disease outbreak
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DOI：
10.1128/jcm.37.12.3883-3887.1999
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发表时间：
1999-12-01
影响因子：
9.4
通讯作者：
Maiden, MCJ
中科院分区：
文献类型：
--
作者：
Feavers, IM;Gray, SJ;Maiden, MCJ
采用多位点序列分型和抗原基因测序对英国一所大学暴发的脑膜炎球菌病进行了调查。获得的数据显示，在疫情爆发期间，学生群体中存在五种不同的ET-37复合物脑膜炎奈瑟菌菌株，其中三种菌株与侵袭性疾病无关，两种不同的菌株引起了侵袭性疾病，包括几例死亡。该疾病群集的最初病例是由一种毒株引起的，这种毒株与导致至少两起后续病例和两起远离该大学的病例的毒株不同，这两起病例在流行病学上与此次暴发有关。这些观察结果与脉冲场凝胶电泳数据一致，但仅凭序列数据就足以确定与该疾病群有关的菌株。核苷酸序列数据的解释比指纹图谱的解释更直接，序列数据提供了分离株间遗传差异的信息。
Multilocus sequence typing and antigen gene sequencing were used to investigate an outbreak of meningococcal disease in a university in the United Kingdom. The data obtained showed that five distinct Neisseria meningitidis strains belonging to the ET-37 complex were present in the student population during the outbreak Three of these strains were not associated with invasive disease, and two distinct strains caused invasive disease, including several fatalities. The initial case of the disease cluster was caused by a strain distinct from that responsible for at least two subsequent cases and two cases remote from the university, which were epidemiologically linked to the outbreak. These observations were consistent with pulsed-field gel electrophoresis data, but the sequence data alone were sufficient to resolve the strains involved in the disease cluster. Interpretation of the nucleotide sequence data was more straightforward than interpretation of the fingerprint patterns, and the sequence data provided information on the genetic differences among the isolates.