Large TCR Diversity of Virus-Specific CD8 T Cells Provides the Mechanistic Basis for Massive TCR Renewal after Antigen Exposure
Large TCR Diversity of Virus-Specific CD8 T Cells Provides the Mechanistic Basis for Massive TCR Renewal after Antigen Exposure
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DOI：
10.4049/jimmunol.1003309
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发表时间：
2011-06-15
影响因子：
4.4
通讯作者：
Pantaleo, Giuseppe
中科院分区：
文献类型：
--
作者：
Miconnet, Isabelle;Marrau, Angelique;Pantaleo, Giuseppe
通过锚定 PCR 对病毒特异性 CD8 T 细胞群进行的体外分析表明，CD8 TCR 库的寡克隆性（每个表位有 7 至 9 个克隆型）比之前想象的要少。在当前的研究中，通过评估来自 27 名健康供体的体外分离的 CMV 和 EBV 特异性 CD8 T 细胞的总体 TCR β 链可变区使用情况以及 CDR3 区域来研究 TCR 多样性。大多数单一病毒表位特异的克隆型的平均数量在 14 到 77 之间。在 HIV-1 慢性感染条件下（即，在病毒复制受到抑制的患者以及治疗中断和 Ag 重新暴露后），CD8 TCR 库的变化也进行了纵向评估。结果表明，大幅更新（
Ex vivo analysis of virus-specific CD8 T cell populations by anchored PCR has shown that the CD8 TCR repertoire was less oligoclonal (seven to nine clonotypes per individual epitope) than previously thought. In the current study, TCR diversity was investigated by assessing both the overall TCR beta-chain variable regions usage as well as the CDR3 regions in ex vivo-isolated CMV- and EBV-specific CD8 T cells from 27 healthy donors. The average number of clonotypes specific to most single viral epitopes comprised between 14 and 77. Changes in the CD8 TCR repertoire were also longitudinally assessed under conditions of HIV-1 chronic infection (i.e., in patients with suppressed virus replication and after treatment interruption and Ag re-exposure). The results showed that a large renewal (