Activation of lymphokine genes during stimulation of cloned T cells.
Activation of lymphokine genes during stimulation of cloned T cells.
复制标题
刺激克隆 T 细胞期间淋巴因子基因的激活。
DOI：
10.1002/eji.1830161211
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发表时间：
1986
影响因子：
5.4
通讯作者：
Fitch,FW
中科院分区：
文献类型：
--
作者：
Herold,KC;Lancki,DW;Dunn,DE;Arai,K;Fitch,FW
为了研究T淋巴细胞产生淋巴因子的调节，我们通过检测克隆的小鼠T淋巴细胞在刺激后淋巴因子基因的水平来表征T细胞中淋巴因子基因的激活。维持培养7天后，未检测到淋巴因子基因的表达。用刀豆蛋白A刺激辅助性T淋巴细胞L2和AD9.1后，淋巴因子mRNA在43小时内出现，达到峰值，然后消失。单个刺激事件可诱导IL-2、IL-3和干扰素-γ的mRNA表达。T辅助淋巴细胞在6h达到最高水平，但也可迟至18h达到最高水平，淋巴因子基因协同表达，但在这些克隆细胞中，IL2mRNA水平似乎低于其他两种mRNAs。上清液中的淋巴因子滴度与mRNA的出现相平行，但IL2滴度在12h后开始下降，这可能是由于激活的细胞利用了这种淋巴因子。在溶细胞T淋巴细胞，L3，在凝集素或克隆型抗体刺激后，发现了定性相似的动力学，与抗体一起出现峰值的时间较晚(18h)。这些研究表明：(A)单个刺激事件以协调的方式激活T细胞的淋巴因子基因；(B)上清液中淋巴因子的出现代表了这些蛋白质的新合成，但上清液中淋巴因子的水平反映了生产和利用率，以及(C)刺激时暴露于IL2对其他淋巴因子的产生并不是必需的。
To study the regulation of lymphokine production by T lymphocytes, we have characterized the activation of lymphokine genes in T cells by measuring the levels of lymphokine mRNA in cloned murine T lymphocytes after stimulation. Lymphokine mRNA was not detected in cells taken after seven days of maintenance culture. Following stimulation of T helper lymphocytes L2 and AD9.1 with concanavalin A, lymphokine mRNA appeared, reached peak levels and disappeared over a 43‐h time period. A single stimulation event resulted in the induction of mRNA for interleukin 2 (IL2), IL3 and interferon gamma. Maximal mRNA levels were generally found at 6 h in the T helper lymphocytes, but could occur as late as 18 h. The lymphokine genes were expressed coordinately; however, in these cloned cells, IL2 mRNA levels appeared to be lower than the other two mRNAs. Lymphokine titers in the supernatant fluids paralleled the appearance of mRNA but IL2 titers began to fall after 12 h probably because of utilization of this lymphokine by the activated cells. In the cytolytic T lymphocyte, L3, qualitatively similar kinetics were found after stimulation by lectin or a clonotypic antibody with peak mRNA levels occurring later (18 h) with the antibody. These studies indicate (a) a single stimulating event activates the lymphokine genes of T cells in a coordinate manner; (b) the appearance of the lymphokines in supernatant fluids representsde novosynthesis of these proteins but the levels of lymphokines measured in supernatant fluids reflects both production and utilization rates, and (c) exposure to IL2 at the time of stimulation is not essential for the production of other lymphokines.