Taste Responsiveness to Sweeteners Is Resistant to Elevations in Plasma Leptin
Taste Responsiveness to Sweeteners Is Resistant to Elevations in Plasma Leptin
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DOI：
10.1093/chemse/bju075
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发表时间：
2015-05-01
期刊：
影响因子：
3.5
通讯作者：
Munger, Steven D.
中科院分区：
文献类型：
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作者：
Glendinning, John I.;Elson, Amanda E. T.;Munger, Steven D.
小鼠血浆瘦素与甜味之间的关系尚不确定。两项研究报告称，血浆瘦素的升高会降低对甜味剂的反应，而另一项研究则发现它们会增强对蔗糖的反应。我们评估了血浆瘦素对 C57BL/6J (B6) 和瘦素缺陷 ob/ob 小鼠甜味的影响。尽管小鼠在 2 型味觉细胞中选择性表达长型瘦素受体 (LepRb)，但瘦素未能激活味觉细胞中关键的瘦素信号蛋白 STAT3。同样，我们没有观察到腹膜内 (i.p.) 瘦素治疗对 B6 或 ob/ob 小鼠鼓索神经对甜味剂的反应有任何影响。最后，瘦素治疗对 B6 小鼠对几种蔗糖浓度的初始舔舐反应没有影响。我们证实，无论一天中的时间、生理状态或体重如何，基础血浆瘦素水平均不超过 10 ng/mL，这表明味觉细胞 LepRb 在我们的研究中并未对瘦素脱敏。此外，ip。瘦素注射产生的血浆瘦素水平超过了之前报道的产生味觉影响的水平。我们得出的结论是，血浆瘦素对甜味剂味觉反应的任何影响都是微妙的，并且仅在特定的实验条件下才会显现出来。
There is uncertainty about the relationship between plasma leptin and sweet taste in mice. Whereas 2 studies have reported that elevations in plasma leptin diminish responsiveness to sweeteners, another found that they enhanced responsiveness to sucrose. We evaluated the impact of plasma leptin on sweet taste in C57BL/6J (B6) and leptin-deficient ob/ob mice. Although mice expressed the long-form leptin receptor (LepRb) selectively in Type 2 taste cells, leptin failed to activate a critical leptin-signaling protein, STAT3, in taste cells. Similarly, we did not observe any impact of intraperitoneal (i.p.) leptin treatment on chorda tympani nerve responses to sweeteners in B6 or ob/ob mice. Finally, there was no effect of leptin treatment on initial licking responses to several sucrose concentrations in B6 mice. We confirmed that basal plasma leptin levels did not exceed 10 ng/mL, regardless of time of day, physiological state, or body weight, suggesting that taste cell LepRb were not desensitized to leptin in our studies. Furthermore, i.p. leptin injections produced plasma leptin levels that exceeded those previously reported to exert taste effects. We conclude that any effect of plasma leptin on taste responsiveness to sweeteners is subtle and manifests itself only under specific experimental conditions.