Transesterification reaction between medium- and long-chain fatty acid triglycerides using surfactant-modified lipase
Transesterification reaction between medium- and long-chain fatty acid triglycerides using surfactant-modified lipase
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DOI：
10.1002/(sici)1097-0290(20000305)67:5
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发表时间：
2000-03-05
影响因子：
3.8
通讯作者：
Mukataka, S
中科院分区：
文献类型：
--
作者：
Mogi, K;Nakajima, M;Mukataka, S
研究了脂肪酶与表面活性剂山梨糖醇酐单硬脂酸酯复合物经表面活性剂改性后，在非溶剂体系中中中长链脂肪酸甘油三酯（MCT）与长链脂肪酸甘油三酯（LCT）的酯交换反应。在48小时的反应期后获得74%的转化率，并且甘油三酯组成由1，3-随机2-随机随机模型很好地描述。MCT和LCT之间的酯交换反应遵循简单的动力学模型，并且可以用一个参数来模拟MCT和LCT含量的变化。研究了改性脂肪酶的水活度A（w）、反应体系的含水量和反应温度对反应速率的影响。改性脂肪酶A（w）为0.35，反应体系的水含量为0.09wt%时，活性最高。在60 ℃以下不发生失活，然而，在70 ℃以上的温度下，活性降低。(C)John Wiley & Sons，Inc.
Transesterification between medium-chain fatty acid triglycerides (MCT) and long-chain fatty acid triglycerides (LCT) in a nonsolvent system was investigated using surfactant modified lipase which is a complex of lipase, Rhizopus japonicus and surfactant, sorbitan monostearate. 74% conversion of was obtained after a 48-h reaction period, and the triglyceride composition was well described by the 1,3-random 2-random stochastic model. The transesterification reaction between MCT and LCT closely followed the simple kinetic model, and the change in MCT and LCT contents could be simulated using one parameter. The effects of the water activity (A(w)) of modified lipase, the water content of the reaction system and the reaction temperature on the reaction rate were studied. modified lipase A(w) of 0.35 and a water content of the reaction system at 0.09 wt % showed the highest activity. Inactivation did not occur below 60 degrees C, however, the activity decreased at temperatures over 70 degrees C. (C) 2000 John Wiley & Sons, Inc.