ASSOCIATION WITH HELA-CELLS OF CAMPYLOBACTER-JEJUNI AND CAMPYLOBACTER-COLI ISOLATED FROM HUMAN FECES
ASSOCIATION WITH HELA-CELLS OF CAMPYLOBACTER-JEJUNI AND CAMPYLOBACTER-COLI ISOLATED FROM HUMAN FECES
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DOI：
10.1128/iai.54.2.283-287.1986
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发表时间：
1986-11-01
影响因子：
3.1
通讯作者：
PFISTER, A
中科院分区：
文献类型：
--
作者：
FAUCHERE, JL;ROSENAU, A;PFISTER, A
我们建立了一种快速的体外试验来确定空肠弯曲菌和大肠埃希氏菌与HeLa细胞的相关性。关联度表示为关联度指数(AI)中与一个细胞相关联的细菌数量的加权平均值。使用随机选择的四个菌株重复测试六次，以检查AI的重复性。平均数和标准差为7.3。+-。1.2、6.8、+-。0.9、1.8。+-。1.2和0.1.+-。0.2。结果可靠的实验条件已标准化。在42株来自人类粪便的菌株中，出现了两组：22株非联合菌株(52%)，AI值在0.0到2.1(平均+-)之间。SD，0.5。+-。联合菌株20株(48%)，AI值在3.5~8.3(平均+-)之间。SD，6.2.+-。1.4)。在这42株细菌中，有17株是临床记录的。合并菌株感染的患者腹泻发生率高于非侵袭性菌株感染患者(7/7比3/10，P=0.01)。感染联合菌株的患者发热发生率更高(6/7比2/10，P=0.03)。用庆大霉素杀灭胞外细菌后的透射电子显微镜和活菌计数支持相关的弯曲杆菌属的事实。附着在细胞膜上，并内化到细胞质液泡中。所描述的试验似乎是一种方便和快速地估计给定菌株的致病性的方法。
We developed a rapid in vitro test for determining the association of Campylobacter jejuni and C. coli with HeLa cells. Association was expressed as a weighted mean of the number of bacteria associated with one cell in an association index (AI). The reproducibility of the AI was checked by repeating the test six times, using four strains chosen at random. Means and standard deviations of the means were 7.3 .+-. 1.2, 6.8 .+-. 0.9, 1.8 .+-. 1.2, and 0.1 .+-. 0.2. The experimental conditions for which the results are reliable have been standardized. Among 42 strains from human feces, two groups appeared: for 22 nonassociative strains (52%), AI values ranged from 0.0 to 2.1 (mean .+-. SD, 0.5 .+-. 0.6); for 20 associative strains (48%), AI values ranged from 3.5 to 8.3 (mean .+-. SD, 6.2 .+-. 1.4). Of these 42 strains, 17 were clinically documented. Diarrhea occurred more frequently in patients infected with associative strains than in those infected with noninvasive strains (7/7 versus 3/10, P = 0.01). Fever also occurred more frequently in patients infected with associative strains (6/7 versus 2/10, P = 0.03). Transmission electron microscopy and viable counts made after killing of extracellular bacteria by gentamicin support the fact that associated Campylobacter spp. are adherent to the cell membrane and are internalized into cytoplasmic vacuoles. The described test seems to be a convenient and rapid method for estimating the pathogenicity of a given strain.