Caveolin-1 and Hsp70 interaction in microdissected proximal tubules from spontaneously hypertensive rats as an effect of Losartan
Caveolin-1 and Hsp70 interaction in microdissected proximal tubules from spontaneously hypertensive rats as an effect of Losartan
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DOI：
10.1097/hjh.0b013e328332b778
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发表时间：
2010-01-01
影响因子：
4.9
通讯作者：
Valles, Patricia G.
中科院分区：
文献类型：
--
作者：
Bocanegra, Victoria;Manucha, Walter;Valles, Patricia G.
研究背景小窝蛋白是AT（1）受体通过胞吐途径转运所必需的。Hsp 70分子伴侣通过与蛋白质的相互作用调节多种信号通路。方法在自发性高血压大鼠（SHR）近曲小管中，我们研究了AT（1）受体拮抗剂Losartan对Caveolin-1和Hsp 70蛋白结合的影响。还研究了Hsp 70参与氯沙坦氧化应激调节。5周龄的SHR被随机分为接受氯沙坦（40 mg/kg/d）（SHRLos）或不接受治疗（SHRH 2 O），持续6周。Wistar-Kyoto大鼠（WKY）血压正常controls.Results通过蛋白质印迹法，小窝蛋白-1的相对丰度是2倍高显微解剖近端小管膜组分从治疗SHR vs.WKYH2O。与WKYH_2 O相比，WKYH_2 O可上调SHRLos近曲小管膜Hsp 70的表达（P <0.05），并可上调SHRLos近曲小管膜Hsp 70的表达（P <0.05）。
Background Caveolin is required to traffic the AT(1) receptor through the exocytic pathway. The chaperone Hsp70 regulates a diverse set of signaling pathways via their interactions with proteins.Method Here we examined the AT(1) receptor antagonist Losartan effect on caveolin-1 and Hsp70 protein association in spontaneously hypertensive rat (SHR) proximal tubules. Hsp70 involvement in Losartan oxidative stress regulation was also studied. Five-week-old SHRs were randomized for receiving Losartan (40 mg/kg per day) (SHRLos) or no treatment (SHRH2O) during 6 weeks. Wistar-Kyoto rats (WKY) were normotensive controls.Results By western blotting, the relative abundance of caveolin-1 was two-fold higher in microdissected proximal tubule membrane fractions from treated SHRs vs. WKYH2O. Hsp70 membrane translocation was demonstrated in SHRLos through out the up-regulation of Hsp70 expression in microdissected proximal tubule membrane fractions when compared with WKYH2O (P