Pumpkin CmHKT1;1 Controls Shoot Na⁺ Accumulation via Limiting Na⁺ Transport from Rootstock to Scion in Grafted Cucumber.
Pumpkin CmHKT1;1 Controls Shoot Na⁺ Accumulation via Limiting Na⁺ Transport from Rootstock to Scion in Grafted Cucumber.
复制标题
DOI：
10.3390/ijms19092648
复制
发表时间：
2018-09-06
影响因子：
5.6
通讯作者：
Bie Z
中科院分区：
文献类型：
--
作者：
Sun J;Cao H;Cheng J;He X;Sohail H;Niu M;Huang Y;Bie Z
土壤盐分对作物的生长和产量产生不利影响，包括世界上最重要的蔬菜之一黄瓜。以耐盐南瓜为砧木进行嫁接，通过限制Na+从南瓜砧木向黄瓜接穗的转运，有效地促进了黄瓜在不同盐分条件下的生长。高亲和力钾转运蛋白（HKT）是植物体内Na+长距离转运的关键，但南瓜HKT在黄瓜嫁接植株Na+长距离转运过程中的作用尚不清楚。在这项工作中，我们的特点是CmHKT 1;1作为HKT基因家族的成员在南瓜，并观察到一个明显的上调CmHKT 1;1在根在NaCl胁迫条件下。在酵母突变体中的异源表达分析表明，CmHKT 1;1是一个Na+选择性转运蛋白。在烟草表皮细胞中的瞬时表达和原位杂交结果表明，CmHKT 1;1定位于质膜，并在根中柱中优先表达。此外，CmHKT 1;1在黄瓜中的异位表达降低了植株地上部的Na+积累。最后，CmHKT 1;1转基因株系作为砧木降低了野生型芽中的Na+含量。这些结果表明，CmHKT 1;1通过限制Na+从砧木向接穗的运输在嫁接黄瓜的耐盐性中起着关键作用，并且可以进一步用于瓜类作物的耐盐性工程。
Soil salinity adversely affects the growth and yield of crops, including cucumber, one of the most important vegetables in the world. Grafting with salt-tolerant pumpkin as the rootstock effectively improves the growth of cucumber under different salt conditions by limiting Na+ transport from the pumpkin rootstock to the cucumber scion. High-affinity potassium transporters (HKTs) are crucial for the long distance transport of Na+ in plants, but the function of pumpkin HKTs in this process of grafted cucumber plants remains unclear. In this work, we have characterized CmHKT1;1 as a member of the HKT gene family in Cucurbita moschata and observed an obvious upregulation of CmHKT1;1 in roots under NaCl stress conditions. Heterologous expression analyses in yeast mutants indicated that CmHKT1;1 is a Na+-selective transporter. The transient expression in tobacco epidermal cells and in situ hybridization showed CmHKT1;1 localization at plasma membrane, and preferential expression in root stele. Moreover, ectopic expression of CmHKT1;1 in cucumber decreased the Na+ accumulation in the plants shoots. Finally, the CmHKT1;1 transgenic line as the rootstock decreased the Na+ content in the wild type shoots. These findings suggest that CmHKT1;1 plays a key role in the salt tolerance of grafted cucumber by limiting Na+ transport from the rootstock to the scion and can further be useful for engineering salt tolerance in cucurbit crops.
登录
查看更多内容
影响因子：
7.2
作者：
Byrt, Caitlin Siobhan;Xu, Bo;Gilliham, Matthew
通讯作者：
Gilliham, Matthew
影响因子：
4.9
作者：
Chen, GX;Fu, XP;Sagi, M
通讯作者：
Sagi, M
影响因子：
4.2
作者：
BANULS, J;PRIMOMILLO, E
通讯作者：
PRIMOMILLO, E
影响因子：
20.5
作者：
Horie, Tomoaki;Hauser, Felix;Schroeder, Julian I.
通讯作者：
Schroeder, Julian I.
影响因子：
7.2
作者：
Horie, T;Yoshida, K;Shinmyo, A
通讯作者：
Shinmyo, A