S-Nitrosothiol analysis via photolysis and amperometric nitric oxide detection in a microfluidic device.
S-Nitrosothiol analysis via photolysis and amperometric nitric oxide detection in a microfluidic device.
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通过光解和微流体装置中的光解和安培一氧化氮检测进行S-亚硝基硫醇分析。
DOI：
10.1021/ac503220z
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发表时间：
2015-03-17
影响因子：
7.4
通讯作者：
Schoenfisch MH
中科院分区：
文献类型：
--
作者：
Hunter RA;Schoenfisch MH
将530 nm的发光二极管耦合到微流控传感器上，以促进亚硝硫醇(即S-亚硝基谷胱甘肽、S-亚硝半胱氨酸和S-亚硝白蛋白)的光解和生成的一氧化氮(NO)的安培检测。这种配置允许最大的灵敏度和多功能性，同时限制紫外线引起的硝酸盐分解的潜在干扰。与散装溶液中总S亚硫醇含量的类似测量相比，使用微流控平台可以显著提高在磷酸盐缓冲盐水和血浆中的分析性能(根据亚硫醇类型的不同，灵敏度提高6-20倍)。此外，能够将样本量从毫升减少到微升，从而提高了临床实用价值。为了证明它在生物学分析中的潜力，该设备被用来测量健康猪模型血管中基础亚硝硫醇的水平。
A 530 nm light emitting diode was coupled to a microfluidic sensor to facilitate photolysis of nitrosothiols (i.e., S-nitrosoglutathione, S-nitrosocysteine, and S-nitrosoalbumin) and amperometric detection of the resulting nitric oxide (NO). This configuration allowed for maximum sensitivity and versatility while limiting potential interference from nitrate decomposition caused by ultraviolet light. Compared to similar measurements of total S-nitrosothiol content in bulk solution, use of the microfluidic platform permitted significantly enhanced analytical performance in both phosphate buffered saline and plasma (6–20× improvement in sensitivity depending on nitrosothiol type). Additionally, the ability to reduce sample volumes from milliliters to microliters provides increased clinical utility. To demonstrate its potential for biological analysis, this device was used to measure basal nitrosothiol levels in the vasculature of a healthy porcine model.