Evolution of switchgrass (Panicum virgatum L.) based on sequences of the nuclear gene encoding plastid acetyl-CoA carboxylase
Evolution of switchgrass (Panicum virgatum L.) based on sequences of the nuclear gene encoding plastid acetyl-CoA carboxylase
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DOI：
10.1016/s0168-9452(02)00327-8
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发表时间：
2003-01-01
期刊：
影响因子：
5.2
通讯作者：
Gornicki, P
中科院分区：
文献类型：
--
作者：
Huang, SX;Su, XJ;Gornicki, P
柳枝菊是一种多年生草本植物，原产于美国和加拿大，具有多种倍性水平和生态型。本研究利用DNA序列比对的方法，对6个柳枝柳品种的胞质乙酰-辅酶A羧基酶编码基因的遗传变异进行了研究，以确定不同种群之间的亲缘关系。在27个基因的1828个核苷酸序列中(外显子696个核苷酸，内含子1132个核苷酸)，外显子有4个多态位点，内含子有18个多态位点和4个内含子。四倍体和八倍体柳枝条的同源基因组之间以及在低地和旱地柳枝条生态型之间存在着非常密切的关系。根据早先确定的小麦族的分子时钟，基因组分化时间和建立各种当代柳枝柳谱系的多倍化事件的时间被估计为不到200万年前。(C)2002爱思唯尔科学爱尔兰有限公司。保留所有权利。
Switchgrass, Panicum virgatum, is a perennial grass native to the USA and Canada with an array of ploidy levels and ecotypes. In this study, genetic variation in the nuclear gene encoding plastid acetyl-CoA carboxylase from six switchgrass cultivars was investigated with the goal of defining relationships among these populations using DNA sequence comparisons. Four polymorphic sites were found in exons and 18 polymorphic sites plus four indels were found in introns within a 1828-nucleotide sequence alignment (696 nucleotides in exons and 1132 nucleotides in introns) of 27 genes. The homoeologous genomes of tetraploid and octaploid switchgrass are very closely related to each other, and among and between lowland and upland switchgrass ecotypes. Based on the molecular clock determined earlier for the Triticeae tribe, the genome divergence time and the time of the polyploidization events which established various contemporary switchgrass lineages was estimated to be less than 2 million years ago. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.