TITRATION AND CHARACTERIZATION OF 2 RHESUS-DERIVED SIVMAC CHALLENGE STOCKS
TITRATION AND CHARACTERIZATION OF 2 RHESUS-DERIVED SIVMAC CHALLENGE STOCKS
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DOI：
10.1089/aid.1994.10.213
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发表时间：
1994-02-01
影响因子：
1.5
通讯作者：
EDDY, GA
中科院分区：
文献类型：
--
作者：
LEWIS, MG;BELLAH, S;EDDY, GA
猴免疫缺陷病毒感染猕猴是人类免疫缺陷病毒感染人类的模型。体内滴定的SIV储备液对于利用该模型进行疫苗开发至关重要。在人细胞中制备的一些疫苗引起的抗人细胞抗体和在人细胞中产生的疫苗的相关保护作用表明需要新的猕猴源SIV储备。在这里，我们描述了两个股票的SIVmac的主要恒河猴细胞中产生的滴定和表征。第一种病毒是从猕猴251的组织中分离的SIVmac251，第二种病毒是命名为SIVmac239的分子克隆。通过静脉接种，滴定每种病毒贮备液的50%恒河猴感染剂量（MID（50））。另外5只猕猴接种10 MID（50）SIVmac251原种，并跟踪疾病结果。所有5只猴在攻毒后14天均出现抗原血症。五只猴子中有两只产生了很强的抗SIV体液免疫，而三只产生了很少或没有体液免疫。如先前所观察到的，疾病进展的快速性与缺乏强抗体应答相关。3只体液免疫力低下的动物在攻毒后7个月内死亡，表现为抗原血症、恶病质、低蛋白血症、低白蛋白血症、体重减轻和顽固性腹泻，同时维持其循环CD4数量。一只动物在1.5岁时死于更典型的猿类艾滋病。
Simian immunodeficiency virus infection of macaques is a model for human immunodeficiency virus infection of humans. In vivo-titrated stocks of SIV are essential for the utilization of this model for vaccine development. The elicitation of anti-human cell antibodies by some vaccines prepared in human cells and the related protective effects of the vaccine produced in human cells suggest a need for new macaque-derived SIV stocks. Here we describe the titration and characterization of two stocks of SIVmac that were produced in primary rhesus macaque cells. The first virus is SIVmac251, isolated from tissues of macaque 251, and the second is a molecular clone designated as SIVmac239. A 50% rhesus monkey infectious dose (MID(50)) was titrated for each virus stock by intravenous inoculation. An additional five macaques were inoculated with 10 MID(50) of the SIVmac251 stock and were followed for disease outcome. All five monkeys developed antigenemia by 14 days postchallenge. Two of the five monkeys developed strong anti-SIV humoral immunity, whereas three developed little or no humoral immunity. As has been observed previously, the rapidity of disease progression correlated with the lack of a strong antibody response. The three animals with low humoral immunity died within 7 months of challenge, with antigenemia, cachexia, hypoproteinemia, hypoalbuminemia, weight loss, and intractable diarrhea, while maintaining their circulating CD4 numbers. One animal died at 1.5 years of more typical simian AIDS.