Impact of the acute ammonia toxicity on phyllosoma Stage I of a slipper lobster, Ibacus novemdentatus and its recoverability from the ammonia exposure
Impact of the acute ammonia toxicity on phyllosoma Stage I of a slipper lobster, Ibacus novemdentatus and its recoverability from the ammonia exposure
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急性氨毒性对 Ibacus novemdentatus 叶状体 I 期的影响及其从氨暴露中的恢复性
DOI：
10.1111/are.15415
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发表时间：
2021
影响因子：
2
通讯作者：
Wakabayashi Kaori
中科院分区：
文献类型：
--
作者：
Quan Nguyen Hong;Suzuki Kenta;Nogata Yasuyuki;Wakabayashi Kaori
滑虾是世界上重要的经济甲壳类动物之一，有望成为一种潜在的水产养殖物种。本研究旨在探讨氨对中华绒螯蟹（Ibacus novemdentatus）第一阶段幼体的急性毒性，并探讨其在氨暴露后能否恢复。六种不同浓度的总氨（TAN）暴露（0、1、2、4、8和16 mg L-1）对10只幼虫的平均致死浓度表明，TAN 96 h（LC 50 - 96 h）根据幼虫活力波动：2.81 mg L−1（0.21 mg L− 1的未离子化氨，NH3-N），当幼虫在0 mg L− 1的TAN下从孵化到阶段II显示出47.7%的存活率时，96%时为6.88 mg L-1（NH3-N为0.56 mg L-1）。第一阶段的幼虫显示出96%的存活率，表明它们能够从高达8 mg L− 1的TAN暴露中恢复24 h，然后达到第二阶段。通过使用0.1的施用系数，在本急性毒性试验中估计的LC 50 - 96 h表明，在长期饲养该物种幼虫时，NH3-N应维持在0.05 mg L− 1或更低。
Slipper lobsters are one of the commercially important crustaceans worldwide and are expected to be a potential aquaculture species. This study aimed to determine the acute toxicity of ammonia for Stage I larvae of a slipper lobster,Ibacus novemdentatus, and to examine whether they could recover after the ammonia exposure. Total ammonia (TAN) exposures at six different concentrations (0, 1, 2, 4, 8, and 16 mg L−1) to 10 larvae in each treatment demonstrated that the mean lethal concentration of TAN for 96‐h (LC50‐96 h) fluctuated in accordance with the larval viability: 2.81 mg L−1(0.21 mg L−1of unionized ammonia, NH3‐N) when the larvae showed the survival rate of 47.7% from hatching to the Stage II at 0 mg L−1of TAN, and 6.88 mg L−1(0.56 mg L−1of NH3‐N) when 96%. Stage I larvae showing the survival rates of 96% revealed their ability to recover from the 24‐h TAN exposure at up to 8 mg L−1and then reached Stage II. By using an application factor of 0.1, the estimated LC50‐96 h in the present acute toxicity test suggests that the NH3‐N should be maintained at 0.05 mg L−1or lower in a long‐term rearing of this species larvae.