Successful culture techniques for Helicobacter species: general culture techniques for Helicobacter pylori.
Successful culture techniques for Helicobacter species: general culture techniques for Helicobacter pylori.
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DOI：
10.1007/978-1-62703-005-2_4
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发表时间：
2012
影响因子：
--
通讯作者：
J. Whitmire;D. Merrell
中科院分区：
文献类型：
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作者：
J. Whitmire;D. Merrell
世界上有一半的人口持续感染幽门螺杆菌。这种感染的慢性化最终导致临床表现从胃炎和消化性溃疡到腺癌和MALT淋巴瘤。由于无法分离和培养这种细菌，在最初观察到Helicobacterspecies后进行的实验室研究受到了极大的阻碍。因此，从该属培养细菌物种的能力是扩大临床知识和开发治疗方法的极其重要的一步。本章介绍了培养H的成功技术。pylorion选择性马血琼脂培养基和布鲁氏菌肉汤液体培养基。此外，还指出了其他螺旋杆菌种的特殊生长要求。
Half of the world’s population is persistently infected withHelicobacter pylori. The chronicity of this infection ultimately elicits clinical manifestations ranging from gastritis and peptic ulcers to adenocarcinoma and MALT lymphoma. Laboratory research following the initial observations ofHelicobacterspecies was greatly hindered by an inability to isolate and culture the bacteria. Thus, the ability to culture bacterial species from this genus is an extremely important step in expanding clinical knowledge and development of therapies. This chapter describes successful techniques for culturingH. pylorion selective horse blood agar media and in Brucella broth liquid media. Additionally, the specific growth requirements of otherHelicobacterspecies are noted.