Relationship between phenolic compounds, anthocyanins content and antioxidant activity in colored barley germplasm
Relationship between phenolic compounds, anthocyanins content and antioxidant activity in colored barley germplasm
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DOI：
10.1021/jf0701943
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发表时间：
2007-06-13
影响因子：
6.1
通讯作者：
Chung, Ill-Min
中科院分区：
文献类型：
--
作者：
Kim, Mi-Jung;Hyun, Jong-Nae;Chung, Ill-Min
大麦及其制品是抗氧化剂的良好来源。本实验旨在检测127种有色大麦品系中酚类化合物、原花青素和花青素的分类及含量，并研究它们与1,1 - 二苯基 - 2 - 苦基肼（DPPH）自由基清除活性的关系。使用比色计将大麦分为七组：有壳（黑色1、黑色2、黑色3和紫色）和无壳（黑色、蓝色和紫色）。采用高效液相色谱法分析酚类化合物和花青素的含量。无壳大麦组酚类化合物的平均含量（268.6微克/克）高于有壳大麦组（207.0微克/克）（P>0.05）。采用改良的香草醛法测定原花青素含量。紫色和蓝色大麦组原花青素的平均含量显著高于黑色组（P<0.05）。花青素含量在13.0至1037.8微克/克之间变化。紫色和蓝色大麦组花青素的平均含量高于黑色组（P<0.05）。紫色大麦组中最常见的花青素是矢车菊素 - 3 - 葡萄糖苷，而飞燕草素 - 3 - 葡萄糖苷是蓝色和黑色组中含量最丰富的花青素。在有色大麦中，DPPH自由基清除活性与酚类化合物和原花青素的含量呈高度正相关。
Barley and its products are good sources of antioxidants. This experiment was conducted to examine the classification and concentration of phenolic compounds, proanthocyanidins, and anthocyanins in 127 lines of colored barley. Their relationship with 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity was also examined. Barley was placed into seven groups using the colorimeter: hulled (black 1, black 2, black 3, and purple) and unhulled (black, blue, and purple). The contents of phenolic compounds and anthocyanins were analyzed by using HPLC. The average content of phenolic compounds in unhulled barley groups (268.6 mu g/g) was higher than that in hulled (207.0 mu g/g) (P > 0.05). The proanthocyanidins content was determined by modified vanillin assay. The average content of proanthocyanidins was significantly higher in purple and blue barley groups compared with black (P < 0.05). The content of anthocyanins varied from 13.0 to 1037.8 mu g/g. Purple and blue barley groups contained higher average contents of anthocyanins than black (P < 0.05). The most common anthocyanin in the purple barley groups was cyanidin 3-glucoside, whereas delphinidin 3-glucoside was the most abundant anthocyanin in the blue and black groups. In colored barley, DPPH radical scavenging activity had high positive correlation to the content of phenolic compounds and proanthocyanidins.