Effect of Momordica saponin- and Cyclocarya paliurus polysaccharide-enriched beverages on oxidative stress and fat accumulation in Caenorhabditis elegans
Effect of Momordica saponin- and Cyclocarya paliurus polysaccharide-enriched beverages on oxidative stress and fat accumulation in Caenorhabditis elegans
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DOI：
10.1002/jsfa.10966
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发表时间：
2020-12-13
影响因子：
4.1
通讯作者：
Chen, Yunjiao
中科院分区：
文献类型：
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作者：
Lin, Chunxiu;Lin, Yizi;Chen, Yunjiao
背景技术青钱柳叶作为中国民间医学中的食药用草药，传统上广泛用于治疗代谢紊乱。蔬菜苦瓜因其对抗肥胖和糖尿病的功效，作为传统药物在全世界范围内食用了数千年。鉴于罗汉果皂苷（MSs）和青蒿多糖（CPPs）的治疗价值，我们独立开发了一种含MSs和CPPs的饮料（MC），对其控制秀丽隐杆线虫氧化应激和肥胖的功效进行了评估。结果首先，我们发现MC可以促进DAF-16的核定位和SOD-3的翻译。进一步探索其抗氧化特性，在线虫中氧化应激副产物活性氧、丙二醛和非酯化脂肪酸均受到显着抑制。此外，与氧化应激相关的疾病（老年色素和神经退行性疾病）造成的损害也得到了减轻。此外，正常和高脂肪模型中的脂肪积累显着减少。最后，MC 的降脂作用可能涉及减少脂滴的大小和数量，而不损害线虫的基本生理功能。结论这些结果提供了有希望的数据，表明 MC 作为一种创新的健康饮料，用于氧化应激和肥胖的药理管理。
BACKGROUND As an edible and medicinal herb in Chinese folk medicine, Cyclocarya paliurus (Batal.) Iljinskaja leaves are traditionally widely used in the treatment of metabolic disorders. The vegetable Momordica charantia L. has been consumed worldwide for thousands of years as a traditional drug due to its activities against obesity and diabetes. In view of the therapeutic value of Momordica saponins (MSs) and C. paliurus polysaccharides (CPPs), an independently developed MSs- and CPPs-containing beverage (MC) was evaluated for its efficacy in controlling oxidative stress and obesity in Caenorhabditis elegans.RESULTS First, we found that MC could promote the nuclear localization of DAF-16 and the translation of SOD-3. Further exploring its antioxidant properties, the oxidative stress by-products reactive oxygen species, malondialdehyde, and nonesterified fatty acids were significantly inhibited in C. elegans. Moreover, damage due to diseases related to oxidative stress (age pigments and neurodegenerative diseases) was alleviated. Furthermore, fat accumulation was significantly reduced in normal and high-fat models. Finally, the lipid-lowering effects of MC might involve reductions in the size and number of lipid droplets without impairing basic physiological functions in C. elegans.CONCLUSION These results provide promising data indicating MC as an innovative health beverage for the pharmacological management of oxidative stress and obesity.