TRANSMITTER LEAKAGE FROM MOTOR-NERVE ENDINGS
TRANSMITTER LEAKAGE FROM MOTOR-NERVE ENDINGS
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DOI：
10.1098/rspb.1977.0029
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发表时间：
1977-01-01
期刊：
影响因子：
--
通讯作者：
MILEDI, R
中科院分区：
文献类型：
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作者：
KATZ, B;MILEDI, R
当抗酯酶处理的青蛙肌肉的终板接受大量离子电渗剂量的箭毒时，在许多纤维中记录到小的局部超极化，平均总计约40μV。这种效应的起源可能是由于细胞质乙酰胆碱 (ACh) 从神经末梢泄漏，在突触间隙中建立了 10-8 M 数量级的 ACh 浓度，并导致终板的微小稳定去极化。据计算，这种稳定的乙酰胆碱泄漏虽然产生了几乎无法检测到的电作用，但可能超过自发量子放电引起的流出量两个数量级，并解释了静息肌肉释放乙酰胆碱的分析量。
When endplates of anti-esterase treated frog muscle were subjected to a massive ionophoretic dose of curare, a small local hyperpolarization was recorded in many fibers, amounting on the average to about 40 .mu.V. The origin of this effect may be attributed to leakage of cytoplasmic acetylcholine (ACh) from nerve terminals, building up an ACh concentration of the order of 10-8 M in the synaptic cleft and causing a minute steady depolarization of the endplate. It was calculated that such a steady leakage of ACh, although producing a barely detectable electrical effort, could exceed the efflux due to spontaneous quantal discharges by 2 orders of magnitude, and account for the assayed amounts of ACh release from resting muscle.