Effects of cell density and conditioned medium on alpha 2A adrenergic receptor density and messenger RNA abundance in HT-29 cells.
Effects of cell density and conditioned medium on alpha 2A adrenergic receptor density and messenger RNA abundance in HT-29 cells.
复制标题
细胞密度和条件培养基对 HT-29 细胞中 α2A 肾上腺素受体密度和信使 RNA 丰度的影响。
DOI：
10.1016/0024-3205(94)90117-1
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发表时间：
1994
期刊：
影响因子：
6.1
通讯作者：
Hoffman,BB
中科院分区：
文献类型：
--
作者：
Sakaue,M;Hoffman,BB
我们研究了HT-29细胞密度与α-2A型肾上腺素能受体数目及基因表达的关系。随着细胞密度从75±25增加到400±73fmol/mg蛋白，α-2a受体表达增加，α-2a受体表达增加约4倍，而β-肌动蛋白表达丰度无明显变化。我们从HT-29细胞融合培养皿中制备了无血清条件培养液(SFCM)，并将其应用于低密度接种的细胞。培养3d后，α-2 9细胞的受体数量增加4.7倍，培养2 4h后，HT2 9细胞的HT2 A受体基因表达水平增加约3倍，但用新鲜培养液透析并不能消除上述作用。这些结果表明，从HT-29细胞分泌的一种物质调节α-2A受体的表达。
We have examined the relationship between cell density and α2A adrenergic receptor number and mRNA in HT-29 cells. α2A receptors increased with increasing cell density from 75±25 to 400±73 fmol/mg protein; α2A receptor mRNA also increased about 4 fold with no change in abundance of β-actin mRNA. We prepared serum-free conditioned medium (SFCM) from confluent dishes of HT-29 cells and applied this SFCM to cells seeded at low density. A 4.7-fold increase in receptor number was observed in HT-29 cells cultured in SFCM for 3 days and α2A receptor mRNA level increased about 3-fold after 24 h. Dialysis of SFCM against fresh growth medium did not abolish these effects. These results suggest that a substance secreted from HT-29 cells regulates expression of α2A receptors.