Overexpression of MtRAV3 enhances osmotic and salt tolerance and inhibits growth of Medicago truncatula.
Overexpression of MtRAV3 enhances osmotic and salt tolerance and inhibits growth of Medicago truncatula.
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DOI：
10.1016/j.plaphy.2021.04.003
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发表时间：
2021-04
期刊：
影响因子：
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通讯作者：
Shumin Wang;Tao Guo;Yixin Shen;Zhen Wang;Junmei Kang;Jiaju Zhang;Fengyan Yi;Qingchuan Yang;R. Long
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文献类型：
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作者：
Shumin Wang;Tao Guo;Yixin Shen;Zhen Wang;Junmei Kang;Jiaju Zhang;Fengyan Yi;Qingchuan Yang;R. Long
与 ABI3/VP1 (RAV) 相关的转录因子在调节植物生长和胁迫耐受性中发挥着重要作用，这些转录因子已在许多植物物种中进行了研究，但在豆科植物中仍然很大程度上未被识别。为了对豆科植物RAV进行功能表征，从豆科植物模式植物Medicagotruncatula中分离出MtRAV3，并通过在M中过表达MtRAV3来研究其功能。特伦卡图拉。表达分析表明MtRAV3受到NaCl和聚乙二醇(PEG)的显着诱导。MtRAV3过表达增强了转基因M的耐受性。截短甘露醇、干旱和盐胁迫，并诱导逆境相关基因的表达，包括MtWRKY76、MtMYB61、冷驯化特异性蛋白31（MtCAS31）、替代氧化酶1（MtAOX1）和乙烯反应因子1（MtERF1）。在盐胁迫和干旱胁迫下，MtRAV3过表达植物的叶片相对电解质渗漏量均低于野生型植物，叶绿素含量较高。蒺藜具有矮化、花期晚、分枝多、花叶器官较小等表型。进一步研究发现，与矮化和分枝表型相关的DWARF14（MtD14）、CAROTENOIDCLEAVAGEDIOXYGENASES7（MtCCD7）和GA3-氧化酶1（MtGA3ox1）的表达水平也明显降低。如MtGA3ox1’(MTR_1g011580)、GA20-氧化酶1(MtGA20ox1)、MtGA20ox1’(MTR_1g102070)和GA20-氧化酶2(MtGA20ox2)参与赤霉素(GAs)途径。总的来说，我们的结果表明MtRAV3在逆境响应和植物生长中发挥着重要作用，是M.中的一个多功能基因。 truncatula，为苜蓿（Medicago sativa）遗传改良提供参考。
Related to ABI3/VP1 (RAV) transcription factors play important roles in regulating plant growth and stress tolerance, which have been studied in many plant species, but have remained largely unidentified in legumes. To functionally characterize RAV in legumes,MtRAV3from legume model plantMedicagotruncatulawas isolated and its function was investigated by overexpressingMtRAV3inM. truncatula. Expression analysis demonstrated thatMtRAV3was markedly induced by NaCl and polyethylene glycol (PEG).MtRAV3overexpression enhanced tolerance of transgenicM. truncatulato mannitol, drought and salt stresses, and induced the expression of adversity-related genes, includingMtWRKY76,MtMYB61,cold-acclimation specific protein 31(MtCAS31), alternative oxidase1(MtAOX1) andethylene response factor 1(MtERF1). There were lower relative electrolyte leakage and higher chlorophyll content of leaves in theMtRAV3overexpression plants than in wild type plants under both salt and drought stress.MtRAV3overexpressionM. truncatulawere featured by some phenotypes of dwarfing, late flowering, more branches, smaller flower and leaf organs. Further investigations showed that the expression levels ofDWARF14(MtD14), CAROTENOIDCLEAVAGEDIOXYGENASES7 (MtCCD7) andGA3-oxidase1(MtGA3ox1), which related to dwarf and branch phenotype, were obviously reduced, as well asMtGA3ox1’(MTR_1g011580),GA20-oxidase1(MtGA20ox1),MtGA20ox1’(MTR_1g102070) andGA20-oxidase2(MtGA20ox2) involved in gibberellins (GAs) pathway. Overall, our results revealed thatMtRAV3exerted an important role in adversity response and plant growth, was a multifunctional gene inM. truncatula, which provided reference for genetic improvement of alfalfa (Medicago sativa).