Enhanced Genetic Characterization of Influenza A(H3N2) Viruses and Vaccine Effectiveness by Genetic Group, 2014-2015
Enhanced Genetic Characterization of Influenza A(H3N2) Viruses and Vaccine Effectiveness by Genetic Group, 2014-2015
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DOI：
10.1093/infdis/jiw181
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发表时间：
2016-10-01
影响因子：
6.4
通讯作者：
Fry, Alicia M.
中科院分区：
文献类型：
--
作者：
Flannery, Brendan;Zimmerman, Richard K.;Fry, Alicia M.
背景。在2014-2015年美国流感季节，采用扩展的流行甲型流感（H3N2）病毒遗传特征来评估甲型流感（H3N2）病毒遗传变异性对流感疫苗有效性（VE）的影响。基于血凝素（HA）基因序列，采用一种新型焦磷酸测序方法，从美国流感疫苗有效性网络站点登记的患者中确定甲型流感（H3N2）病毒的遗传群。采用检测阴性设计，比较甲型流感（H3N2）病毒感染患者和未感染患者的疫苗接种情况，估计VE。在9710名入组者中，1868名（19%）对甲型流感（H3N2）病毒检测呈阳性；对1397株病毒的遗传鉴定显示，1134株（81%）属于抗原漂移甲型流感（H3N2）病毒的1 HA遗传群（3C.2a）。2014-2015年甲型流感（H3N2）病毒基因组不同，流感疫苗接种对抗原漂移型甲型流感（H3N2）病毒3C组引起的疾病的有效性从1%（95%可信区间[CI]， -14%至14%）不等。2a病毒对疫苗样甲型流感（H3N2）病毒组3C引起的疾病的预防率为44% （95% CI, 16%-63%）。3 b viruses.Conclusions。2014-2015年流感疫苗接种效果因甲型流感（H3N2）病毒基因群而异。与抗原漂移相关的HA基因变化与VE降低有关。
Background. During the 2014-2015 US influenza season, expanded genetic characterization of circulating influenza A(H3N2) viruses was used to assess the impact of the genetic variability of influenza A(H3N2) viruses on influenza vaccine effectiveness (VE).Methods. A novel pyrosequencing assay was used to determine genetic group, based on hemagglutinin (HA) gene sequences, of influenza A(H3N2) viruses from patients enrolled at US Influenza Vaccine Effectiveness Network sites. VE was estimated using a test-negative design comparing vaccination among patients infected with influenza A(H3N2) viruses and uninfected patients.Results. Among 9710 enrollees, 1868 (19%) tested positive for influenza A(H3N2) virus; genetic characterization of 1397 viruses showed that 1134 (81%) belonged to 1 HA genetic group (3C.2a) of antigenically drifted influenza A(H3N2) viruses. Effectiveness of 2014-2015 influenza vaccination varied by influenza A(H3N2) virus genetic group from 1% (95% confidence interval [CI], -14% to 14%) against illness caused by antigenically drifted influenza A(H3N2) virus group 3C.2a viruses versus 44% (95% CI, 16%-63%) against illness caused by vaccine-like influenza A(H3N2) virus group 3C.3b viruses.Conclusions. Effectiveness of 2014-2015 influenza vaccination varied by genetic group of influenza A(H3N2) virus. Changes in HA genes related to antigenic drift were associated with reduced VE.