Cutting Edge: A Newly Identified Response Element in the CD95 Ligand Promoter Contributes to Optimal Inducibility in Activated T Lymphocytes
Cutting Edge: A Newly Identified Response Element in the CD95 Ligand Promoter Contributes to Optimal Inducibility in Activated T Lymphocytes
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最前沿：CD95 配体启动子中新鉴定的响应元件有助于激活 T 淋巴细胞的最佳诱导能力
作者：
L. Norian;K. Latinis;G. Koretzky
活化T淋巴细胞上CD95配体的诱导表达有助于细胞毒性效应机制和外周T细胞稳态。为了更好地理解调节这种表达的转录事件，我们已经检查了CD95配体启动子，以确定T细胞刺激后其诱导活性所需的区域。我们在这里报告的启动子内的一个新的响应元件的识别，需要其在激活的Jurkat T细胞的最佳功能。该区域与活化T细胞核提取物中所含的蛋白质结合，但不与静息T细胞结合。该序列的多聚化独立地驱动响应于T细胞活化的转录，而其突变显著降低诱导型启动子活性。最后，我们提供的证据表明，T细胞活化诱导的转录的CD95配体基因的调节协调的反应元件连同两个先前定义的网站的活化T细胞的核因子（NFAT）。
Inducible expression of CD95 ligand on activated T lymphocytes contributes to both cytotoxic effector mechanisms and peripheral T cell homeostasis. To understand better the transcriptional events that regulate this expression, we have examined the CD95 ligand promoter to determine which regions are required for its induced activity following T cell stimulation. We report here the identification of a new response element within the promoter that is required for its optimal function in activated Jurkat T cells. This region is bound by proteins contained in nuclear extracts of activated, but not resting, T cells. Multimerization of this sequence independently drives transcription in response to T cell activation, while mutation of it substantially decreases inducible promoter activity. Finally, we provide evidence that T cell activation-induced transcription of the CD95 ligand gene is regulated coordinately by this response element together with two previously defined sites for nuclear factor of activated T cells (NFAT).
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通过 TCR 介导的信号事件调节 CD95 (Fas) 配体表达。
DOI：
--
发表时间：
1997
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Latinis,KM;Carr,LL;Peterson,EJ;Norian,LA;Eliason,SL;Koretzky,GA
通讯作者：
Koretzky,GA
T 淋巴细胞中的信号传导事件导致细胞激活或程序性细胞死亡。
DOI：
10.1006/clin.1996.4315
发表时间：
1997
期刊：
Clinical immunology and immunopathology
影响因子：
--
作者：
Musci,MA;Latinis,KM;Koretzky,GA
通讯作者：
Koretzky,GA
小鼠 T 细胞杂交瘤中激活诱导的细胞死亡。
影响因子：
4.4
作者：
Brunner,T;Yoo,NJ;LaFace,D;Ware,CF;Green,DR
通讯作者：
Green,DR
影响因子：
56.9
作者：
MCCAFFREY, PG;LUO, C;HOGAN, PG
通讯作者：
HOGAN, PG