Inhibition of Escherichia coli fructose-1,6-bisphosphatase by fructose 2,6-bisphosphate.
Inhibition of Escherichia coli fructose-1,6-bisphosphatase by fructose 2,6-bisphosphate.
复制标题
果糖 2,6-二磷酸对大肠杆菌果糖-1,6-二磷酸酶的抑制作用。
DOI：
10.1016/0006-291x(84)90888-x
复制
发表时间：
1984
影响因子：
3.1
通讯作者：
Rittenhouse,J
中科院分区：
文献类型：
--
作者：
Marcus,F;Edelstein,I;Rittenhouse,J
2，6-二磷酸果糖是一种有效的果糖-1，6-二磷酸酶抑制剂，也是大肠杆菌酶的抑制剂。在抑制剂存在下的底物饱和曲线是S形的，并且在低底物浓度下的抑制比在高底物浓度下强得多。当底物浓度为20 μM时，4.8 μM果糖2，6-二磷酸酶抑制率为50%，AMP（Kj= 16 μM）抑制大肠杆菌果糖-1，6-二磷酸酶，磷酸烯醇丙酮酸释放AMP抑制。然而，无论是AMP的抑制，也不是其释放磷酸烯醇式丙酮酸果糖2，6-二磷酸的存在下受到影响。所获得的结果，连同以前的观察，提供了进一步的证据，果糖2，6-二磷酸果糖-1，6-二磷酸酶活性位点的相互作用。
Fructose 2,6-bisphosphate, a potent inhibitor of fructose-1,6-bisphosphatases, was found to be an inhibitor of theEscherichia colienzyme. The substrate saturation curves in the presence of inhibitor were sigmoidal and the inhibition was much stronger at low than at high substrate concentrations. At a substrate concentration of 20 μM, 50% inhibition was observed at 4.8 μM fructose 2,6-bisphosphate.Escherichia colifructose-1,6-bisphosphatase was inhibited by AMP (Kj= 16 μM) and phosphoenolpyruvate caused release of AMP inhibition. However, neither AMP inhibition nor its release by phosphoenolpyruvate was affected by the presence of fructose 2,6-bisphosphate. The results obtained, together with previous observations, provide further evidence for the fructose 2,6-bisphosphate-fructose-1,6-bisphosphatase active site interaction.