Aptamer TY04 inhibits the growth of multiple myeloma cells via cell cycle arrest
Aptamer TY04 inhibits the growth of multiple myeloma cells via cell cycle arrest
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适体TY04通过细胞周期阻滞抑制多发性骨髓瘤细胞的生长
DOI：
10.1007/s13277-014-1920-2
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发表时间：
2014-08-01
期刊：
影响因子：
--
通讯作者：
Tan, Weihong
中科院分区：
文献类型：
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作者：
Dai, Hongjuan;Ye, Mao;Tan, Weihong
适体TY04是单链DNA。然而，其生物学功能尚未阐明。我们发现TY04特异性结合多发性骨髓瘤细胞MM.1S， MM.1S细胞表面的一些膜蛋白构成了TY04的靶分子。TY04抑制多发性骨髓瘤细胞系的生长，诱导有丝分裂的细胞周期阻滞，导致双核细胞的显著积累。在TY04治疗后，CDK1和cyclin B1的表达同时增加。此外，TY04处理也导致γ-微管蛋白显著下调。考虑到适体的独特优势，TY04作为治疗多发性骨髓瘤的候选药物具有很大的潜力。
The aptamer TY04 is a single-stranded DNA. However, its biological function has not been elucidated. Here, we found that TY04 specifically bound to multiple myeloma cells MM.1S, and some membrane proteins on the surface of MM.1S cells constituted the target molecules of TY04. TY04 inhibited the growth of multiple myeloma cell lines, induced cell cycle arrest in mitosis, and resulted in a significant accumulation of binucleated cells. Following TY04 treatment, a concomitant increase in CDK1 and cyclin B1 expression occurred. In addition, TY04 treatment also resulted in a significant downregulation of γ-tubulin. Considering the unique advantages of aptamers, TY04 shows great potential as a drug candidate to treat multiple myeloma.