Measurement of relative avidity of antibodies reactive with Porphyromonas (Bacteroides) gingivalis in the sera of subjects having adult periodontitis.
Measurement of relative avidity of antibodies reactive with Porphyromonas (Bacteroides) gingivalis in the sera of subjects having adult periodontitis.
复制标题
测量患有成人牙周炎的受试者血清中与牙龈卟啉单胞菌（拟杆菌）反应的抗体的相对亲合力。
DOI：
10.1111/j.1600-0765.1991.tb01641.x
复制
发表时间：
1991
影响因子：
3.5
通讯作者：
Lee,SW
中科院分区：
文献类型：
--
作者：
Lopatin,DE;LaBelle,D;Lee,SW
用硫氰酸铵-解离酶联免疫吸附试验测定了15例成人牙周炎患者和15例健康人血清中牙龈卟啉单胞菌(类杆菌)抗体的相对亲和力。将分级浓度的硫氰酸铵加入血清的单一稀释液中，以解离低亲和力抗体结合顶端。牙周炎。使结合(吸光度)降低50%的硫氰酸盐浓度称为该血清的ID50。当检测到Ig G类抗体时，患者血清中抗牙龈假单胞菌抗体的ID50显著升高(0.96Mvs0.71M；p<；0.01，学生t检验)。相反，当检测IgM类抗体时，P的ID50在患者和对照组之间没有发现显著差异。牙周炎(0.54米比0.53米)。病人抗体的ID_(50)值高于健康对照，但与P免疫兔的抗体相比，差异不显著。来自其他人类研究的ID50值表明，成年人通常会产生非常低亲和力的抗体TOP。牙周炎。提示低亲和力抗体的存在与牙周病相关的病理改变有关。
Relative avidities of antibodies toPorphyromonas (Bacteroides) gingivalisin the sera of 15 patients having adult periodontitis and 15 healthy subjects were evaluated using an ammonium thiocyanate‐dissociated ELISA. Graded concentrations of ammonium thiocyanate were added to a single dilution of serum in order to dissociate low avidity antibody binding toP. gingivalis. The concentration of thiocyanate resulting in 50% reduction in binding (absorbance) was termed the ID50for that serum. When IgG‐class antibodies were examined, the ID50of anti‐P. gingivalisantibodies in the sera of patients was significantly elevated (0.96M vs 0.71M; p<0.01, Student's t‐test). In contrast, when IgM‐class antibodies were examined no significant differences in ID50between patients and controls were found forP. gingivalis(0.54M vs 0.53M). While the ID50values of patient antibodies were found to be elevated relative to those of healthy controls, comparison with antibodies from rabbits immunized withP. gingivalisand with ID50values from other human studies suggests that adult humans, in general, produce very low‐avidity antibodies toP. gingivalis. It is suggested that the presence of low‐avidity antibodies contributes to the pathology associated with periodontal disease.