The effects of pH on DNA methylation state:: In vitro and post-mortem brain studies
The effects of pH on DNA methylation state:: In vitro and post-mortem brain studies
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DOI：
10.1016/j.jneumeth.2008.06.027
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发表时间：
2008-09-15
影响因子：
3
通讯作者：
Turecki, Gustavo
中科院分区：
文献类型：
--
作者：
Ernst, Carl;McGowan, Patrick O.;Turecki, Gustavo
脑组织DNA甲基化状态的检测已成为表观遗传学和精神病理学研究的热点之一。已知大脑中的pH效应会影响基因转录，但pH对DNA甲基化状态的影响尚不清楚。Vie在体外使用人工甲基化质粒证明DNA甲基化状态即使在极端pH条件下也保持稳定。接下来，使用来自人类DNA的两个不同的基因组区域，我们使用具有不同pH水平的受试者评估来自皮质和皮质下脑区域的甲基化状态。DNA甲基化状态与pH值无相关性，提示死后脑组织DNA甲基化状态稳定。皇冠版权所有（C）2008年出版的爱思唯尔B.V.保留所有权利。
Assessment of methylation state of DNA extracted from brain is becoming one of the most investigated issues in the study of epigenetics and psychopathology. pH effects in brain are known to affect gene transcription, though pH effects on DNA methylation state are unknown. Vie demonstrate in vitro using an artificially methylated plasmid that DNA methylation state remains stable, even under extreme pH conditions. Next, using two different genomic regions from human DNA, we assess methylation state from both cortical and sub-cortical brain regions using subjects with varying pH levels. No correlation was found between DNA methylation state and pH. These results suggest that DNA methylation state is stable in post-mortem brain. Crown Copyright (C) 2008 Published by Elsevier B.V. All rights reserved.