Effects of dietary non-protein energy source levels on growth performance, body composition and lipid metabolism in herbivorous grass carp (Ctenopharyngodon idella Val.)
Effects of dietary non-protein energy source levels on growth performance, body composition and lipid metabolism in herbivorous grass carp (Ctenopharyngodon idella Val.)
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日粮非蛋白能源水平对草食性草鱼生长性能、身体成分和脂质代谢的影响（Ctenopharyngodon idella Val.）
DOI：
10.1111/are.12275
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发表时间：
2015-05-01
影响因子：
2
通讯作者：
Li, Bin
中科院分区：
文献类型：
--
作者：
Guo, Xiaoze;Liang, Xu-Fang;Li, Bin
研究了不同非蛋白质能量源对草鱼生长、组织脂肪积累及脂肪代谢相关基因表达的影响。三个试验组分别饲喂4种等氮（300 gkg（-1））、4种非蛋白质能量水平（6.52kJg（-1）对照组、5.32kJg（-1）高CEL组、8.46kJg（-1）高CHO组和8.53kJg（-1）高LIP组）的试验饲料9周。提高非蛋白质能量水平对草鱼的生长没有促进作用，高能量水平的饲料降低了草鱼的生长。高胆固醇饮食倾向于诱导高肝体指数，肝脏脂肪和糖原含量高。然而，高脂饮食引起高肠系膜脂肪指数，但不增加肝脏脂肪。高胆固醇饮食组肝脏脂肪生成酶的mRNA丰度和活性显著增加，而高脂饮食组则相反。肝脏过氧化物酶体增殖物激活受体（PPAR）和肠系膜脂肪组织中的PPAR在高CEL饮食组中上调。脂蛋白脂酶（LPL）基因在高脂饲料组的肝脏和肠系膜脂肪组织中的表达显著增加，而在高脂饲料组的肝脏中LPL基因的表达上调，而在肠系膜脂肪组织中LPL基因的表达下调。这些结果表明，膳食非蛋白质能源的增加改变了脂质代谢和增加脂质沉积的基因表达。
A study was conducted to determine the effects of dietary non-protein energy sources on growth, tissue lipid accumulation and lipid metabolism-related genes expression of grass carp. Triplicate groups of fish were fed for 9weeks on four isonitrogenous (300gkg(-1)) experimental diets with four levels of non-protein energy (6.52kJg(-1) control diet, 5.32kJg(-1) high-CEL diet, 8.46kJg(-1) high-CHO diet and 8.53kJg(-1) high-LIP diet respectively). Increasing dietary non-protein energy source levels did not improve the growth, and the high-CEL diet reduced the growth of grass carp. The high-CHO diet tended to induce high hepatosomatic index, with high fat and glycogen content of liver. However, the high-LIP diet caused the high mesenteric fat index, but did not increase liver fat. The mRNA abundance and activities of hepatic lipogenic enzymes were significantly increased in the high-CHO diet group, whereas the opposite tendencies were observed in the high-LIP diet group. Peroxisome proliferator-actived receptor- (PPAR) in liver and PPAR in mesenteric adipose tissue were up-regulated in the high-CEL diet group. Lipoprotein lipase (LPL) gene expression was significantly increased both in liver and mesenteric adipose tissue of fish fed the high-LIP diet, while the LPL gene expression was up-regulated in liver but down-regulated in mesenteric adipose tissue of fish fed the high-CEL diet. These findings suggest that an increase in dietary non-protein energy sources alters the genes expression of lipid metabolism and increased lipid deposition.