Systemic control of protein synthesis through sequestration of translation and ribosome biogenesis factors during severe heat stress
Systemic control of protein synthesis through sequestration of translation and ribosome biogenesis factors during severe heat stress
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DOI：
10.1016/j.febslet.2015.10.010
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发表时间：
2015-11-30
期刊：
影响因子：
3.5
通讯作者：
Bukau, Bernd
中科院分区：
文献类型：
--
作者：
Cherkasov, Valeria;Grousl, Tomas;Bukau, Bernd
环境应激导致蛋白质被隔离成不溶性沉积物，包括含有 mRNA 和多种翻译因子的细胞质应激颗粒 (SG)。在这里，我们通过 SG 共定位筛选和不溶性蛋白质组分的蛋白质组学分析，系统地鉴定了 46 摄氏度下酿酒酵母中隔离的蛋白质。我们鉴定了新的 SG 成分，包括必需的氨酰基-tRNA 合成酶。此外，我们发现了含有核糖体生物发生因子的细胞核相关沉积物。我们的研究表明，通过热休克诱导的蛋白质隔离，细胞质蛋白质合成和核核糖体产生在多个水平上下调。 (C) 2015 年欧洲生化学会联合会。由 Elsevier B.V. 出版。保留所有权利。
Environmental stress causes the sequestration of proteins into insoluble deposits including cytoplasmic stress granules (SGs), containing mRNA and a variety of translation factors. Here we systematically identified proteins sequestered in Saccharomyces cerevisiae at 46 degrees C by a SG co-localization screen and proteomic analysis of insoluble protein fractions. We identified novel SG components including essential aminoacyl-tRNA synthetases. Moreover, we discovered nucleus-associated deposits containing ribosome biogenesis factors. Our study suggests downregulation of cytosolic protein synthesis and nuclear ribosome production at multiple levels through heat shock induced protein sequestrations. (C) 2015 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.