RESTRICTED HELPER FUNCTION OF F1 HYBRID T-CELLS POSITIVELY SELECTED TO HETEROLOGOUS ERYTHROCYTES IN IRRADIATED PARENTAL STRAIN MICE .1. FAILURE TO COLLABORATE WITH B-CELLS OF OPPOSITE PARENTAL STRAIN NOT ASSOCIATED WITH ACTIVE SUPPRESSION
RESTRICTED HELPER FUNCTION OF F1 HYBRID T-CELLS POSITIVELY SELECTED TO HETEROLOGOUS ERYTHROCYTES IN IRRADIATED PARENTAL STRAIN MICE .1. FAILURE TO COLLABORATE WITH B-CELLS OF OPPOSITE PARENTAL STRAIN NOT ASSOCIATED WITH ACTIVE SUPPRESSION
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DOI：
10.1084/jem.147.4.1142
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发表时间：
1978-01-01
影响因子：
15.3
通讯作者：
SPRENT, J
中科院分区：
文献类型：
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作者：
SPRENT, J
将未引发的(CBA×C57BL/6)F1淋巴结T[胸腺衍生]细胞与绵羊红细胞(SRC)一起转移至重度辐射的F1或亲本品系小鼠中，并在5天后从受体的胸导管淋巴或脾中回收。为了研究它们的辅助功能，将收获的 F1 T 细胞与抗原一起转移到受辐射的 F1 小鼠中，加上来自 2 个亲本品系或 F1 小鼠的 B [骨髓来源] 细胞。 F1小鼠中激活的F1 T细胞对所有3个B细胞群产生高Ig[免疫球蛋白]M和IgG抗SRC反应。 1 个亲本品系小鼠中激活的 F1 T 细胞与该品系的 B 细胞合作良好，但与相反品系的 B 细胞合作不佳。主动抑制被认为不太可能解释这一结果，因为在 F1 B 细胞中发现了良好的反应，另外的实验表明，通过补充注射在 F1 小鼠中激活的 F1 T 细胞，可以将相对亲本品系的 B 细胞的不良反应（相当于未引发的 F1 T 细胞产生的反应）转化为高反应。该现象对于用于激活的抗原是特异性的（用马红细胞进行了十字交叉实验）；反映在血清血凝素水平和脾斑形成细胞的数量上；也适用于 (DBA/2 × C57BL/6)F1 T 细胞的类似激活；并且可以通过在相对亲本品系的腹膜渗出细胞存在的情况下激活 1 亲本品系小鼠的 F1 T 细胞来预防。提出了这样的假设：F1 T 细胞包含 2 个离散的抗原反应细胞亚群，每个亚群都受到 2 个不同水平的限制：在 T 巨噬细胞相互作用期间和在 T-B 协作期间。当 F1 T 细胞在亲本菌株环境中被抗原激活时，抗辐射巨噬细胞仅激活 2 个 T 细胞亚群中的 1 个。该亚组能够与用于激活的菌株的 B 细胞（以及 F1 B 细胞）合作，但不能与相反亲本菌株的 B 细胞合作。 T 细胞的另一个亚群仍处于未引发（未激活）状态。
Unprimed (CBA .times. C57BL/6)F1 lymph node T [thymus derived] cells were transferred with sheep erythrocytes (SRC) into heavily irradiated F1 or parental strain mice and recovered from thoracic duct lymph or spleens of the recipients 5 days later. To study their helper function, the harvested F1 T cells were transferred with antigen into irradiated F1 mice plus B [bone marrow derived] cells from either the 2 parental strains or from F1 mice. F1 T cells activated in F1 mice gave high Ig[immunoglobulin]M and IgG anti-SRC responses with all 3 populations of B cells. F1 T cells activated in mice of 1 parental strain collaborated well with B cells of this strain, but poorly with B cells of the opposite strain. Active suppression was considered an unlikely explanation for this result since good responses were found with F1 B cells, and additional experiments showed that the poor response with B cells of the opposite parental strain (which was equivalent to that produced by unprimed F1 T cells) could be converted to a high response by a supplemental injection of F1 T cells activated in F1 mice. The phenomenon was specific for the antigen used for activation (criss-cross experiments were performed with horse erythrocytes); was reflected in levels of serum hemagglutinins and in numbers of splenic plaque-forming cells; applied also to comparable activation of (DBA/2 .times. C57BL/6)F1 T cells; and could be prevented by activating F1 T cells in mice of 1 parental strain in the presence of peritoneal exudate cells of the opposite parental strain. The hypothesis was advanced that F1 T cells contain 2 discrete subpopulations of antigen-reactive cells, each subject to restrictions acting at 2 different levels: during T-macrophage interactions and during T-B collaboration. When F1 T cells are activated to antigen in a parental strain environment, radioresistant macrophages activate only 1 of the 2 subgroups of T cells. This subgroup is able to collaborate with B cells of the strain used for activation(and with F1 B cells) but not with B cells of the opposite parental strain. The other subgroup of T cells remains in an unprimed (nonactivated) state.