Chemical Functionalization of Bioceramics To Enhance Endothelial Cells Adhesion for Tissue Engineering
Chemical Functionalization of Bioceramics To Enhance Endothelial Cells Adhesion for Tissue Engineering
复制标题
DOI：
10.1021/jm301092r
复制
发表时间：
2012-09-27
影响因子：
7.3
通讯作者：
Gerber-Lemaire, Sandrine
中科院分区：
文献类型：
--
作者：
Borcard, Francoise;Staedler, Davide;Gerber-Lemaire, Sandrine
为了控制人内皮细胞和人血清蛋白对不同组成的生物陶瓷的选择性粘附，设计、合成并表征了含有环状精氨酸-甘氨酸-天冬氨酸（RGD）肽、四乙二醇间隔基和没食子酸酯部分的多功能配体。证明了该配体与氧化铝基、羟基磷灰石基和磷酸钙基生物陶瓷的结合。这种配体的生物陶瓷的共轭诱导的人血清蛋白的非选择性和整合素选择性结合的减少，而人内皮细胞的结合和粘附增强，依赖于特定的生物陶瓷。
To control the selective adhesion of human endothelial cells and human serum proteins to bioceramics of different compositions, a multifunctional ligand containing a cyclic arginine-glycine-aspartate (RGD) peptide, a tetraethylene glycol spacer, and a gallate moiety was designed, synthesized, and characterized. The binding of this ligand to alumina-based, hydroxyapatite-based, and calcium phosphate-based bioceramics was demonstrated. The conjugation of this ligand to the bioceramics induced a decrease in the nonselective and integrin-selective binding of human serum proteins, whereas the binding and adhesion of human endothelial cells was enhanced, dependent on the particular bioceramics.