Loss of Bcl-2 expression correlates with tumour recurrence in colorectal cancer
Loss of Bcl-2 expression correlates with tumour recurrence in colorectal cancer
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DOI：
10.1136/gut.43.3.383
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发表时间：
1998-09-01
期刊：
影响因子：
24.5
通讯作者：
Talbot, IC
中科院分区：
文献类型：
--
作者：
Ilyas, M;Hao, XP;Talbot, IC
目的-探讨Bcl-2和p53在结直肠癌中的免疫组化表达与肿瘤术后复发的关系。方法-66例Dukes' B期结直肠癌进行了研究。所有肿瘤均为中度分化，组织学上显示切除边缘清晰。使用p53和Bcl-2的单克隆抗体对福尔马林固定的石蜡包埋的组织进行免疫组织化学。分别评估Bcl-2染色的相对染色强度和阳性肿瘤细胞的百分比，并给出结合两个因素的最终评分。仅根据阳性肿瘤细胞的数量评估p53染色。将肿瘤复发病例的免疫染色模式与无肿瘤复发病例进行比较结果：Bcl-2评分与肿瘤复发之间存在统计学显著负相关，（复发性疾病患者的Bcl-2评分中位数为6（四分位距（IQR）2-9）;无复发患者的Bcl-2评分中位数为8（IQR 6-10）; p = 0.03）。当单独检查时，表达强度和阳性肿瘤细胞的百分比与肿瘤复发显著相关（每种情况下p = 0.04）。有p53染色和肿瘤recursion.Conclusion之间没有关联结果表明，当控制分化，Bcl-2表达是一个预后标志物，并可能是有用的作为一个诊断测试在临床决策。
Aims - To investigate the association between immunohistochemical expression of Bcl-2 and p53 in colorectal cancer and tumour recurrence following surgery.Methods - Sixty six cases of Dukes' B colorectal carcinoma were studied. All tumours were moderately differentiated and were shown to be histologically clear of the resection margins. Immunohistochemistry was performed on formalin fixed paraffin wax embedded tissue using monoclonal antibodies for p53 and Bcl-2. The Bcl-2 staining was assessed separately for relative intensity of staining and percentage of positive tumour cells and given a final score which combined the two factors. The p53 staining was assessed on number of positive tumour cells only. The patterns of immunostaining of those cases in which there had been tumour recurrence were compared with those cases in which there was no tumour recurrence (controls).Results - A statistically significant inverse association was found between Bcl-2 score and tumour recurrence (median Bcl-2 score of 6 (interquartile range (IQR) 2-9) in patients with recurrent disease; median Bcl-2 score of 8 (IQR 6-10) in those without recurrence; p = 0.03). When examined separately, both the intensity of expression and percentage of positive tumour cells were significantly associated with tumour recurrence (p = 0.04 in each case). There was no association between p53 staining and tumour recurrence.Conclusion - Results suggest that, when controlled for differentiation, Bcl-2 expression is a prognostic marker and may be useful as an adjunctive test in clinical decision making.