COMPETITION AND DISTRIBUTION STUDIES OF GENETICALLY MARKED STRAINS OF USTILAGO VIOLACEA IN THE SAME HOST PLANT
COMPETITION AND DISTRIBUTION STUDIES OF GENETICALLY MARKED STRAINS OF USTILAGO VIOLACEA IN THE SAME HOST PLANT
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DOI：
10.1086/337162
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发表时间：
1980-01-01
影响因子：
--
通讯作者：
DAY, AW
中科院分区：
文献类型：
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作者：
DAY, AW
用花药黑穗病菌（U.紫罗兰属，含有6个遗传标记菌株，每种交配型3个; 9种可能的双核体可以在植物内形成并竞争。平均而言，从每株植物中取样44个受感染的花蕾，并使用能够鉴定9种可能的双核体的快速筛选技术分析感染双核体的基因型。不同的双核体可以侵入并侵染同一花茎、分支、花梗，甚至很少侵入并侵染同一花药。在177个受感染的花蕾中，90个被野生型+野生型双核体感染，86个被3个野生型+突变体双核体中的1个感染，并且从1朵花分析的9个花药中只有2个被突变体+突变体双核体感染。177朵花中只有2朵含有2个不同的双核体，两个双核体都能穿透同一花药，在同一花药中共同产孢，通常在寄主的一个区域中只有1个双核体占优势。
Four plants of Silene alba were inoculated with a suspension of cells of the anther smut fungus, U. violacea, containing 6 genetically marked strains, 3 of each mating type; 9 possible dikaryons could form and compete within the plant. On average, 44 infected flower buds from each plant were sampled and analyzed for genotype of the infecting dikaryon using rapid screening techniques capable of identifying the 9 possible dikaryons. Different dikaryons could penetrate and infect the same flower shoot, lateral branch, pedicel or, even rarely, the same anther. Of 177 infected flower buds, 90 were infected by a wild-type + wild-type dikaryon, 86 with 1 of 3 wild-type + mutant dikaryons and only 2 anthers of 9 analyzed from 1 flower were infected with a mutant + mutant dikaryon. Only 2 flowers of the 177 contained 2 different dikaryons, both of which could penetrate the same anther, sporulating together within it. Usually only 1 dikaryon comes to predominate in 1 area of the host.