Analysis of human herpesvirus 6 glycoproteins recognized by monoclonal antibody OHV1.
Analysis of human herpesvirus 6 glycoproteins recognized by monoclonal antibody OHV1.
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单克隆抗体 OHV1 识别的人疱疹病毒 6 糖蛋白分析。
DOI：
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发表时间：
1992
影响因子：
3.8
通讯作者：
K. Yamanishi
中科院分区：
文献类型：
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作者：
T. Okuno;H. Shao;H. Asada;K. Shiraki;M. Takahashi;K. Yamanishi
使用抗 HHV-6 单克隆抗体 OHV1 研究了人疱疹病毒 6 (HHV-6) 的病毒特异性糖蛋白 (gp)。对感染细胞提取物的免疫沉淀显示，该抗体在还原条件下可识别 Mrs 为 106K、102K、65K 和 63K 的四种糖基化蛋白 (gps)。然而，在非还原条件下仅检测到 106K (gp106) 和 102K 两种 gps。脉冲追踪实验表明gp65和gp63是gp106和gp102的裂解产物。当用衣霉素处理受感染的细胞时，没有检测到这些 GPS。经内切-N-乙酰氨基葡萄糖苷酶H (endo H)和内切-N-乙酰氨基葡萄糖苷酶F (endo F)处理后，gp106和gp102消失。此外，gp65和gp63不受endo H处理的影响，但对endo F处理敏感。这些数据表明gp106和gp102的糖残基是高甘露糖型N-连接寡糖，而gp65和gp63的糖残基是复合型N-连接寡糖。
A virus-specific glycoprotein (gp) from human herpes-virus 6 (HHV-6) was studied using the anti-HHV-6 monoclonal antibody OHV1. Immunoprecipitation with extracts from infected cells revealed that the antibody recognized four glycosylated proteins (gps) with Mrs of 106K, 102K, 65K and 63K under reducing conditions. However, only two gps, of 106K (gp106) and 102K, were detected under non-reducing conditions. Pulse-chase experiments revealed that gp65 and gp63 were cleavage products of gp106 and gp102. When infected cells were treated with tunicamycin, none of these gps was detected. With endo-beta-N-acetylglucosaminidase H (endo H) and endo-beta-N-acetylglucosaminidase F (endo F) treatment, gp106 and gp102 disappeared. Moreover, gp65 and gp63 were not affected by endo H treatment but were sensitive to endo F treatment. These data suggest that sugar residues of gp106 and gp102 are high-mannose type N-linked oligosaccharides, whereas those of gp65 and gp63 are complex type N-linked oligosaccharides.
3例患者B细胞淋巴瘤组织中人B淋巴细胞病毒（人疱疹病毒6）序列的检测。
影响因子：
11.4
作者：
Josephs,SF;Buchbinder,A;Streicher,HZ;Ablashi,DV;Salahuddin,SZ;Guo,HG;Wong-Staal,F;Cossman,J;Raffeld,M;Sundeen,J
通讯作者：
Sundeen,J