Hydrography shapes bacterial biogeography of the deep Arctic Ocean
Hydrography shapes bacterial biogeography of the deep Arctic Ocean
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DOI：
10.1038/ismej.2009.134
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发表时间：
2010-04-01
期刊：
影响因子：
11
通讯作者：
Lovejoy, Connie
中科院分区：
文献类型：
--
作者：
Galand, Pierre E.;Potvin, Marianne;Lovejoy, Connie
关于海洋微生物是否具有普遍的生物地理分布或生物地理模式，人们一直争论不休，但最近关于微生物生物地理存在的共识正在形成。然而，控制海洋细菌分布的因素仍然知之甚少。在这项研究中，我们将焦磷酸测序和传统的16S rRNA基因Sanger测序相结合，详细描述了来自北冰洋深处的细菌群落。我们的目标是来自三个海洋盆地的三个独立的水团，并表明北冰洋中的细菌具有生物地理学。细菌的生物地理分布可以用北冰洋的水文和随后的水团循环来解释。总的来说，这是对北极深处细菌群落的首次分类描述，揭示了大量存在SAR11 （Alphaproteobacteria）、SAR406、SAR202 （Chloroflexi）和SAR324 （Deltaproteobacteria）集群。在每个群集内，不同水团间特定种型的丰度差异显著。水团可能起到了物理屏障的作用，限制了海洋中细菌的扩散并控制了细菌的多样性。因此，海洋微生物生物地理学涉及的不仅仅是地理距离，因为它还与海洋过程动态相关。我们的海洋尺度研究表明，要充分了解海洋微生物的多样性和功能，必须考虑微生物与物理海洋学之间的耦合。ISME Journal (2010) 4,564 -576；doi: 10.1038 / ismej.2009.134;2009年12月10日在线发布
It has been long debated as to whether marine microorganisms have a ubiquitous distribution or patterns of biogeography, but recently a consensus for the existence of microbial biogeography is emerging. However, the factors controlling the distribution of marine bacteria remain poorly understood. In this study, we combine pyrosequencing and traditional Sanger sequencing of the 16S rRNA gene to describe in detail bacterial communities from the deep Arctic Ocean. We targeted three separate water masses, from three oceanic basins and show that bacteria in the Arctic Ocean have a biogeography. The biogeographical distribution of bacteria was explained by the hydrography of the Arctic Ocean and subsequent circulation of its water masses. Overall, this first taxonomic description of deep Arctic bacteria communities revealed an abundant presence of SAR11 (Alphaproteobacteria), SAR406, SAR202 (Chloroflexi) and SAR324 (Deltaproteobacteria) clusters. Within each cluster, the abundance of specific phylotypes significantly varied among water masses. Water masses probably act as physical barriers limiting the dispersal and controlling the diversity of bacteria in the ocean. Consequently, marine microbial biogeography involves more than geographical distances, as it is also dynamically associated with oceanic processes. Our ocean scale study suggests that it is essential to consider the coupling between microbial and physical oceanography to fully understand the diversity and function of marine microbes. The ISME Journal (2010) 4, 564-576; doi:10.1038/ismej.2009.134; published online 10 December 2009