In vitro bioassay for hypertrehalosemic hormone-dependent trehalose biosynthesis by fat body from adult Blaberus discoidalis cockroaches.
In vitro bioassay for hypertrehalosemic hormone-dependent trehalose biosynthesis by fat body from adult Blaberus discoidalis cockroaches.
复制标题
成年 Blaberus Discoidalis 蟑螂脂肪体高海藻糖激素依赖性海藻糖生物合成的体外生物测定。
DOI：
10.1002/arch.940280402
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Hesson,AS
中科院分区：
文献类型：
--
作者：
Keeley,LL;Sowa,SM;Hesson,AS
一种适合于常规使用的体外生物测定法被开发用于研究高海藻糖激素（HTH）依赖的蟑螂脂肪体的海藻糖生物合成。Blaberus discoidalis脂肪体被分离和分割，以便可以比较来自单个组织的8个匹配块用于多个对照和实验处理。确定了最佳孵育条件和HTH依赖性海藻糖合成的性质。剂量反应研究确定了雄性脂肪体和雌性组织的EC 50分别为0.044 nM HTH和0.16 nM HTH。HTH使雄性脂肪体的海藻糖产量增加3倍，而雌性组织的最大增幅仅为67%，并且在后续研究中仅使用雄性组织。脂肪体仅需暴露于HTH 5分钟即可产生最大海藻糖1小时。孵育培养基中≥ 15 mM海藻糖可抑制海藻糖合成。脂肪体显示出体外海藻糖合成的发育增加，这反映在体内血淋巴海藻糖中。基础和HTH相关海藻糖合成在第0 - 10天较低，到第20天增加3倍，此后较高。这些研究为今后的研究建立了基线数据，以确定参与HTH调节脂肪体代谢的信号转导机制。© 1995 Wiley利斯公司
An in vitro bioassay suitable for routine use to investigate hypertrehalosemic hormone (HTH)‐dependent trehalose biosynthesis was developed for the cockroach fat body.Blaberus discoidalisfat bodies were isolated and divided so that eight matched pieces from a single tissue could be compared for multiple control and experimental treatments. Optimum incubation conditions and the properties of HTH‐dependent trehalose synthesis were determined. Dose‐response studies determined an EC50of 0.044 nM HTH for male fat body and 0.16 nM HTH for female tissue. HTH increased trehalose production by male fat body 3‐fold compared to only a 67% maximum increase by the female tissue, and only the male tissue was used in subsequent studies. Fat body required only 5‐min exposure to HTH for maximum trehalose production for 1 h. Trehalose synthesis was inhibited by ≥ 15 mM trehalose in the incubation medium. The fat body showed a developmental increase in trehalose synthesis in vitro that was reflected by hemolymph trehalose in vivo. Basal and HTH‐related trehalose synthesis were low between days 0 and 10, increased 3‐fold by day 20, and were high thereafter. These studies have established baseline data for future investigations to identify the signal transduction mechanisms involved in HTH regulation of fat body metabolism. © 1995 Wiley‐Liss, Inc.