FUNCTIONAL REGENERATION FOLLOWING SPINAL TRANSECTION DEMONSTRATED IN THE ISOLATED SPINAL-CORD OF THE LARVAL SEA LAMPREY
FUNCTIONAL REGENERATION FOLLOWING SPINAL TRANSECTION DEMONSTRATED IN THE ISOLATED SPINAL-CORD OF THE LARVAL SEA LAMPREY
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DOI：
10.1073/pnas.83.8.2763
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发表时间：
1986-04-01
影响因子：
11.1
通讯作者：
SELZER, ME
中科院分区：
文献类型：
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作者：
COHEN, AH;MACKLER, SA;SELZER, ME
海七鳃鳗幼体的轴突可以在脊髓横断的部位再生，并与一些正常的目标神经元形成功能突触。这些动物恢复了看起来正常的运动，但再生的轴突及其突触连接是否能够在这种行为中发挥功能作用尚不清楚。为了验证这一点，将d -谷氨酸添加到洗浴液中，在孤立的脊髓中诱导“有效”游泳。愈合横断面上下节段的腹侧根放电显示高度锁相。这有力地表明，行为恢复是由再生的功能性突触连接介导的，这些突触连接服务于运动中枢模式发生器的节间协调。
Axons in the larval sea lamprey can regenerate across the site of a spinal cord transection and form functioning synapses with some of their normal target neurons. The animals recover normal-appearing locomotion, but whether the regenerating axons and their synaptic connections are capable of playing a functional role during this behavior is unknown. To test this, "fictive" swimming was induced in the isolated spinal cord by the addition of D-glutamate to the bathing solution. Ventral root discharges of segments above and below a healed transection showed a high degree of phase-locking. This strongly suggests that the behavioral recovery is mediated by regenerated functional synaptic connections subserving intersegmental coordination of the central pattern generator for locomotion.