A differential proteomics study of Caenorhabditis elegans infected with Aeromonas hydrophila
A differential proteomics study of Caenorhabditis elegans infected with Aeromonas hydrophila
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DOI：
10.1016/j.dci.2010.02.003
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发表时间：
2010-06-01
影响因子：
2.9
通讯作者：
Verleyen, Peter
中科院分区：
文献类型：
--
作者：
Bogaerts, Annelies;Temmerman, Liesbet;Verleyen, Peter
脊椎动物和无脊椎动物的天然防御系统之间的惊人相似性，以及秀丽线虫对遗传分析的适应性，使这种自由生活的地面线虫成为细菌致病研究中流行的模式系统。尽管遗传学研究带来了新的见解，显示了免疫反应的诱导性和病原体特异性，但关于感染抵抗的确切机制仍有许多有待发现。本文从不同的角度研究线虫寄主与病原菌的相互作用。我们报道了应用差示凝胶电泳法(DGE)结合质谱学来寻找感染革兰氏阴性杆菌嗜水气单胞菌后在蠕虫中差异合成的蛋白质。鉴于免疫反应的动态性质，在感染后的三个不同时间点对线虫的蛋白质组进行了研究。共鉴定出65个差异蛋白。本研究证实Galectins、C型凝集素和脂结合蛋白参与线虫的免疫。此外，还分离和鉴定了一些未知蛋白质，它们可能代表了蠕虫防御系统的重要角色。这项工作首次表明了在感染过程中蛋白质水平上发生的复杂变化。(C)2010爱思唯尔有限公司。保留所有权利。
The striking similarities between the innate defences of vertebrates and invertebrates as well as the amenability of Caenorhabditis elegans for genetic analysis, have made this free-living ground nematode a popular model system in the study of bacterial pathogenesis. Although genetic studies have brought new insights, showing the inducibility and pathogen-specificity of the immune response, there is still much to be discovered about the exact mechanisms underlying resistance to infection. In this paper a different angle was adopted to study host-pathogen interactions in C. elegans. We report the application of differential gel electrophoresis (DIGE), combined with mass spectrometry to search for proteins that are differentially synthesised in the worm after infection with the gram-negative bacterium Aeromonas hydrophila. Given the dynamic nature of an immune response, the proteome of C. elegans was investigated at three different time-points after infection. A total of 65 differential proteins were identified. This study confirms the involvement of galectins, C-type lectins and lipid binding proteins in the immunity of C. elegans. In addition a number of unknown proteins, which might represent important players of the worm's defence system, were isolated and identified. This work gives a first indication of the complex changes that occur at the protein level during infection. (C) 2010 Elsevier Ltd. All rights reserved.