EGF and FGF-2 responsiveness of rat and mouse neural precursors derived from the embryonic CNS
EGF and FGF-2 responsiveness of rat and mouse neural precursors derived from the embryonic CNS
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DOI：
10.1016/j.brainresbull.2005.08.020
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发表时间：
2005-12-15
影响因子：
3.8
通讯作者：
Rosser, AE
中科院分区：
文献类型：
--
作者：
Kelly, CM;Tyers, P;Rosser, AE
EGF和FGF-2在体外诱导许多不同物种的胚胎神经前体（ENPs）增殖。在这项研究中，我们证明了胚胎年龄是大鼠胚胎神经前体细胞对EGF和/或FGF-2反应的数量和分化潜力的关键决定因素，因为(i)根据分离的胎龄，对两种生长因子（单独或联合）有不同的反应；（ii）当允许分化时，这些细胞产生神经元的能力有时间变化。此外，对于所有胎龄的培养物，有一个明确的衰老模式，当细胞在妊娠早期分离时，培养物扩展时间最长。提示本研究中大鼠ENPs主要由。有限分裂潜能的神经祖细胞而非自我更新的多潜能神经干细胞。相比之下，小鼠的ENPs似乎无限扩大，因此可以进行更长时间的研究，观察生长因子浓度的影响。使用小鼠ENPs评估不同EGF浓度的影响。(c) 2005爱思唯尔公司版权所有。
EGF and FGF-2 induce the proliferation of embryonic neural precursors (ENPs) in vitro from a number of different species. In this study, we demonstrate that embryonic age is a crucial determinant of the number and differentiation potential of rat embryonic neural precursor cells responding to either EGF and/or FGF-2, in that (i) there is a differential response to the two growth factors (both alone and in combination) according to the gestational age of isolation and (ii) when allowed to differentiate, there are temporal changes in the ability of these cells to produce neurons. Furthermore, for cultures of all gestational ages, there is a defined pattern of senescence, with cultures expanding longest when cells are isolated earlier in gestation. The suggestion is that rat ENPs in this study consist predominantly. of neural progenitor cells with limited division potential rather than self-renewing multipotential neural stem cells. In contrast, mouse ENPs appeared to expand indefinitely and thus allow for longer studies to be carried out looking at the effects of growth factor concentrations. The effect of varying the concentration of EGF was assessed using mouse ENPs. (c) 2005 Elsevier Inc. All rights reserved.