Prime genome editing in rice and wheat
Prime genome editing in rice and wheat
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DOI：
10.1038/s41587-020-0455-x
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发表时间：
2020-03-16
影响因子：
46.9
通讯作者：
Gao, Caixia
中科院分区：
文献类型：
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作者：
Lin, Qiupeng;Zong, Yuan;Gao, Caixia
Prime editors是一种CRISPR-Cas9切口酶（H840 A）-逆转录酶融合体，使用prime editing guide RNA（pegRNA）进行编程，可以在哺乳动物细胞中编辑碱基，而无需供体DNA或双链断裂。我们通过密码子、启动子和编辑条件的优化，将主要编辑器用于植物。由此产生的一套植物引物编辑器使水稻和小麦原生质体中的点突变、插入和缺失成为可能。以高达21.8%的频率获得再生的引物编辑的水稻植物。
Prime editors are optimized for use in plants.Prime editors, which are CRISPR-Cas9 nickase (H840A)-reverse transcriptase fusions programmed with prime editing guide RNAs (pegRNAs), can edit bases in mammalian cells without donor DNA or double-strand breaks. We adapted prime editors for use in plants through codon, promoter, and editing-condition optimization. The resulting suite of plant prime editors enable point mutations, insertions and deletions in rice and wheat protoplasts. Regenerated prime-edited rice plants were obtained at frequencies of up to 21.8%.