Primary structure, functional characteristics and tissue expression pattern of human ATA2, a subtype of aminoacid transport system A
Primary structure, functional characteristics and tissue expression pattern of human ATA2, a subtype of aminoacid transport system A
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DOI：
10.1016/s0005-2736(00)00252-2
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发表时间：
2000-07-31
影响因子：
3.4
通讯作者：
Ganapathy, V
中科院分区：
文献类型：
--
作者：
Hatanaka, T;Huang, W;Ganapathy, V
我们在这里报道了负责A系统氨基酸转运活动的蛋白质的一级结构和功能特征，已知这种蛋白质在大多数人类组织中都有表达。这个被指定为ATA的运输机？氨基酸转运蛋白A2是从人肝癌细胞系HepG2中克隆的。人ATA2(HATA2)由506个氨基酸组成，与大鼠ATA2高度同源。HATA2特异的mRNA在人体组织中广泛表达。当在哺乳动物细胞中表达时，hATA2介导依赖于钠离子的α-(甲氨基)异丁酸的转运，α-甲氨基异丁酸是A系统的特定模式底物。该转运体是中性氨基酸的专一性转运体。它对pH敏感，对Li+不耐受。Na+-氨基酸化学计量比为1：1。(C)2000 Elsevier Science B.V.保留所有权利。
We report here on the primary structure and functional characteristics of the protein responsible for the system A amino acid transport activity that is known to be expressed in most human tissues. This transporter, designated ATA? for amino acid transporter A2, was cloned from the human hepatoma cell line HepG2. Human ATA2 (hATA2) consists of 506 amino acids and exhibits a high degree of homology to rat ATA2. hATA2-specific mRNA is ubiquitously expressed in human tissues. When expressed in mammalian cells, hATA2 mediates Na+-dependent transport of alpha-(methylamino)isobutyric acid, a specific model substrate for system A. The transporter is specific for neutral amino acids. It is pH-sensitive and Li+-intolerant. The Na+-amino acid stoichiometry is 1:1. (C) 2000 Elsevier Science B.V. All rights reserved.