PAPER Initial organ distribution and biological safety of Mg2+ released from a Mg alloy implant
PAPER Initial organ distribution and biological safety of Mg2+ released from a Mg alloy implant
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论文 镁合金植入物释放的 Mg2 的初始器官分布和生物安全性
DOI：
10.1088/1748-605x/aaa9d5
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Tachi M.
中科院分区：
文献类型：
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作者：
Sato A1;Shimizu Y;Imai Y;Mukai T;Yamamoto A;Miura C;Muraki K;Sano Y;Ikeo N;Tachi M.
镁合金被认为是很有前途的可生物降解医疗器械材料，然而，镁离子注入后释放的初始效应和分布尚不清楚。本研究使用两种类型的镁合金植入大鼠体内，解决了这一问题。首先进行了体外浸泡实验，以定量测定合金早期释放的镁离子。在此基础上，我们进行了体内实验，将大量合金分别植入大鼠背部皮下1、5、10和2 5h，用电感耦合等离子体质谱检测各器官中镁的蓄积量。在体内，植入1h后，接受植入物的大鼠的血和尿中镁离子浓度高于对照组，但仍在临床可接受的标准范围内。骨组织中镁离子浓度在2 5h组明显高于其他组。我们的结果表明，在大量镁合金种植体的早期阶段，由于体液中镁离子浓度的突然变化，尿液中释放的镁离子的排泄和骨积聚维持了体内的动态平衡。
Magnesium (Mg) alloys are considered promising materials for biodegradable medical devices; however, the initial effects and distribution of released Mg 2+ ions following implantation are unclear. This is addressed in the present study, using two types of Mg alloys implanted into rats. An in vitro immersion test was first carried out to quantify Mg 2+ ions released from the alloys at early stages. Based on these data, we performed an in vivo experiment in which large amounts of alloys were subcutaneously implanted into the backs of rats for 1, 5, 10, and 25 h. Mg 2+ accumulation in organs was measured by inductively coupled plasma mass spectrometry. In vivo, blood and urine Mg 2+ concentrations were higher in rats receiving the implants than in controls after 1 h; however, the levels were within clinically accepted guidelines. The Mg 2+ concentration in bone was significantly higher in the 25 h implanted group than in the other groups. Our results suggest that homeostasis is maintained by urinary excretion and bone accumulation of released Mg 2+ ions in response to sudden changes in Mg 2+ ion concentration in the body fluid in a large number of Mg alloy implants at the early stages.