Circulating tumor cells as an emerging tool in cancer therapy
Circulating tumor cells as an emerging tool in cancer therapy
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DOI：
10.2741/4824
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发表时间：
2020-01-01
影响因子：
3.1
通讯作者：
Kumar, Prashant
中科院分区：
文献类型：
--
作者：
Balakrishnan, Ajay;George, Irene A.;Kumar, Prashant
大多数与癌症相关的死亡与转移有关，在转移过程中癌细胞侵入周围组织，进入（内渗）和退出（外渗）外周循环并播散到远处的器官。循环肿瘤细胞 (CTC) 以单个细胞或肿瘤细胞寡克隆簇以及血小板和淋巴细胞的形式存在于外周血中。 CTC 的检测可以通过基因型来表征肿瘤，并预测预后和对治疗的反应，源自这些细胞的外植体可用于药物筛选。在这篇综述中，我们重点介绍了 CTC 的分离和培养方法及其临床应用。
Majority of the cancer-related deaths are related to metastasis during which cancer cells invade the surrounding tissues, enter (intravasation) and exit (extravasation) the peripheral circulation and seed distant organs. The Circulating Tumor Cells (CTCs) exist in peripheral blood as single cells or as oligoclonal clusters of tumor cells along with platelets and lymphocytes. Detection of CTCs allows characterizing the tumors by their genotype and in predicting the prognosis and response to therapy and explants derived from these cells can be used in drug screening. In this review, we highlight the methods used for isolation and culture of CTCs and their clinical use.