AFLP analysis on genetic relationship among Rheum tanguticum, Rheum palmatum, and Rheum officinale.
AFLP analysis on genetic relationship among Rheum tanguticum, Rheum palmatum, and Rheum officinale.
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发表时间：
2010
影响因子：
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通讯作者：
Suo Fengmei;Song Jingyuan;C. Shilin;W. Yonggang;Mei Hong-fang;M. Eiji;T. Osami;Xie Cai-xiang
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文献类型：
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作者：
Suo Fengmei;Song Jingyuan;C. Shilin;W. Yonggang;Mei Hong-fang;M. Eiji;T. Osami;Xie Cai-xiang
目的探讨3种中药大黄的亲缘关系（唐古特大黄（Rheum tanguticum），掌叶大黄（R.palmatum），方法采用扩增片段长度多态性（AFLP）技术和NTSYSpc. 2.利用11F软件对44个不同种源的野生或栽培药用大黄进行亲缘关系分析，结果共扩增出1 255条谱带，筛选出8条可靠的谱带引物。其中1 209聚类分析结果表明，44种大黄属植物可分为两大类，唐古特大黄属Ⅰ类，掌叶大黄和药用大黄属Ⅱ类。结果表明，不同种源的大黄具有一定的特异性结论与唐古特大黄相比，掌叶大黄与大黄亲缘关系较近，AFLP技术是研究药用大黄亲缘关系的有效方法。
Objective To investigate the genetic relationship of three traditional medicinal rhubarbs(Rheum tanguticum,R.palmatum,and R.officinale.Methods Amplified fragment length polymorphism(AFLP) technology and NTSYSpc-2.11F software were used to study and analyze the affinity between 44 wild or cultivated medicinal rhubarbs from different provenances.Results The 1 255 bands were amplified by polymerase chain reactions with eight screened and reliable primers.Among them 1 209(96.2%) bands showed polymorphic.The 44 different rhubarbs were categorized into two groups by cluster analysis.R.tanguticum belonged to Group Ⅰ,while R.palmatum and R.officinale were clustered to Group Ⅱ.In addition,there were some specific bands among all amplification bands.This result indicated the rhubarbs originated from the different species and provenances had specific DNA bands and may be authenticated according to the characteristics.Conclusion Compared to R.tanguticum,the genetic relationship between R.palmatum and R.offcinale is closer.AFLP Technique is proved to be a very effective method to study the genetic relationships of medicinal rhubarbs.