Circadian transcriptional factor DBP regulates expression of Kiss1 in the anteroventral periventricular nucleus
Circadian transcriptional factor DBP regulates expression of Kiss1 in the anteroventral periventricular nucleus
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DOI：
10.1016/j.mce.2011.03.020
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发表时间：
2011-06-06
影响因子：
4.1
通讯作者：
Adachi, Akihito A.
中科院分区：
文献类型：
--
作者：
Xu, Zhifang;Kaga, Shigehito;Adachi, Akihito A.
Kiss 1在前腹侧室周核（AVPV）及其产物metastin/kisspeptin的表达呈昼夜节律，在晚上达到高峰，与啮齿动物促性腺激素释放激素（GnRH）/促黄体生成激素（LH）高峰的时间有很强的时相关系。在这里，我们报告说，昼夜转录因子，白蛋白D-位点结合蛋白（DBP），能够触发mKiss 1转录通过D盒，这种效果相结合的雌激素受体α（ER α）和它的配体，雌激素。组织学研究表明，成年雌性大鼠AVPV中的一些细胞共表达Dbp和ER α。AVPV中ER α的表达无节律性，而Dbp的mRNA在动情前期有明显的昼夜节律性积累，而在动情间期第1天则无明显的昼夜节律性积累。因此，这些结果表明，DBP和雌激素调节Kiss 1在AVPV中的表达，从而介导GnRH/LH峰。(C)2011爱思唯尔爱尔兰有限公司保留所有权利。
The expression of Kiss1 in the anteroventral periventricular nucleus (AVPV) and its product, metastin/kisspeptin, show a circadian pattern with a peak in the evening, which shows a strong phase relationship with the time of the gonadotropin-releasing hormone (GnRH)/luteinizing hormone (LH) surge in rodents. Here we report that a circadian transcriptional factor, albumin D-site binding protein (Dbp), was able to trigger mKiss1 transcription via the D-box, and this effect was combined with those of estrogen receptor alpha (ER alpha) and its ligand, estrogen. A histological study demonstrated that some cells in the AVPV co-expressed Dbp with ER alpha in adult female rats. Expression of ER alpha was not rhythmic in the AVPV, however, mRNA of Dbp in the AVPV accumulated with a robust diurnal rhythm in proestrus, but not on the first day of diestrus. Thus, these results suggest that Dbp and estrogen regulate the expression of Kiss1 in the AVPV, thereby mediating the GnRH/LH surge. (C) 2011 Elsevier Ireland Ltd. All rights reserved.