The effect of autologous repair and voluntary wheel running on force recovery in a rat model of volumetric muscle loss.
The effect of autologous repair and voluntary wheel running on force recovery in a rat model of volumetric muscle loss.
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DOI：
10.1113/ep089207
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发表时间：
2021-04
影响因子：
2.7
通讯作者：
Wolchok JC
中科院分区：
文献类型：
--
作者：
Washington TA;Perry RA Jr;Kim JT;Haynie WS;Greene NP;Wolchok JC
骨骼肌可以从损伤中再生，但被极端的组织损失所淹没，称为体积肌肉损失（VML）。患有VML的患者不能完全恢复患肢的力输出。最近的研究表明，替代组织（即，签名）到VML缺损部位加上体力活动显示出优化VML后力量恢复的希望。本研究的目的是测量自体修复和自愿车轮运行对VML后力量恢复的影响。切除32只雄性Sprague-Dawley大鼠20%的左侧胫骨前肌（LTA），然后将其替换并缝合到完整肌肉中（自体修复）。右侧胫骨前肌（RTA）作为对侧对照。16只大鼠自由接近转轮（Wheel），而其他16只大鼠留在笼中，转轮锁定（Sed）。在VML后2周和8周，对LTA进行力测试;然后取出肌肉并进行形态学和基因表达分析。受伤后2周，Wheel组的标准化LTA力比Sed组高58%。在VML后8周，Wheel和Sed组之间的LTA力相似，但仍低于未受伤的RTA。VML后2周的基因表达分析显示，与Sed组相比，车轮组的白细胞介素（IL）-1β、IL-6和肿瘤坏死因子α的mRNA含量较低。总的来说，自愿车轮运行促进早期力量恢复，但不足以完全恢复力量。强调早期力量恢复可能是由于更亲再生的微环境。
Skeletal muscle can regenerate from damage but is overwhelmed with extreme tissue loss, known as volumetric muscle loss (VML). Patients suffering from VML do not fully recover force output in the affected limb. Recent studies show that replacement tissue (i.e., autograph) into the VML defect site plus physical activity show promise for optimizing force recovery post-VML. The purpose of this study was to measure the effects of autologous repair and voluntary wheel running on force recovery post-VML. Thirty-two male Sprague–Dawley rats had 20% of their left tibialis anterior (LTA) excised then replaced and sutured into the intact muscle (autologous repair). The right tibialis anterior (RTA) acted as the contralateral control. Sixteen rats were given free access to a running wheel (Wheel) whereas the other 16 remained in a cage with the running wheel locked (Sed). At 2 and 8 weeks post-VML, the LTA underwent force testing; then the muscle was removed and morphological and gene expression analysis was conducted. At 2 weeks post-injury, normalized LTA force was 58% greater in the Wheel group compared to the Sed group. At 8 weeks post-VML, LTA force was similar between the Wheel and Sed groups but was still lower than the uninjured RTA. Gene expression analysis at 2 weeks post-VML showed the wheel groups had lower mRNA content of interleukin (IL)-1β, IL-6 and tumour necrosis factor α compared to the Sed group. Overall, voluntary wheel running promoted early force recovery, but was not sufficient to fully restore force. The accentuated early force recovery is possibly due to a more pro-regenerative microenvironment.